The Divalent Metal Transporter 1 (DMT1) BioAssay™ ELISA Kit (Porcine) is a quantitative sandwich assay for the detection of DMT1 in serum, plasma, tissue homogenates and other biological fluids.||Sensitivity:|0.094ng/ml||Detection Range:|0.156-10ng/ml||Specificity:|This assay has high sensitivity and excellent specificity for detection of porcine DMT1. No significant cross-reactivity or interference between DMT1 and analogues was observed.||Precision:|Intra-Assay: CV<8%|Inter-Assay: CV<10%||Test Principle:|This ELISA kit employs the sandwich enzyme-linked immune-sorbent assay technology. Anti-DMT1 antibody was pre-coated onto 96-well plates. The biotin conjugated anti-DMT1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the DMT1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of DMT1 can be calculated.||Kit Components:|Micro ELISA Plate(Dismountable), 8×12|Lyophilized Standard, 2x vial|Sample / Standard dilution buffer, 1x20ml|Biotin-labeled antibody (Concentrated), 1x120ul|Antibody dilution buffer, 1x10ml|HRP-Streptavidin Conjugate(SABC), 1x120ul|SABC dilution buffer, 1x10ml|TMB substrate, 1x10ml|Stop solution, 1x10ml|Wash buffer (25X), 1x30ml|Plate Sealer, 1x5pieces||Storage and Stability:|Store unopened kit at 4ºC; store at -20ºC once opened. Store unopened standard at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

USBIo全称United States BIOLOGical，位于麻省斯万普斯科特(Swampscott, MA)。全美**的抗原抗体和生化试剂供应商，目前向全球六十余个国家的科研工作者提供约50,000种抗体、抗原和生化试剂，以质量**闻名。主要产品种类包括：抗体、生化试剂、基因克隆相关产品、培养基、生长因子等细胞因子、分子生物学试剂。美国US Biological公司是生物化学和生物学试剂的专业生产商，产品服务于免疫学，分子生物学，分子遗传学，体外诊断，组织培养等科研领域。产品精确度高，质量稳定，产品数量巨大，提供70,000多种抗体、抗原和生化试剂等。

Usbio的生物化学品，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。USBio产品从经济实惠的研究数量到更大的批量可用于工艺开发和生产。我们的客户包括位于美国和国外的大多数*的制药和生物技术公司。

USbiological公司致力于以价值，诚信和真正的个人购买体验降低研究成本。USbiological的生物化学，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。产品可以从负担得起的研究数量到大批量用于工艺开发和生产。

USBio（UnitedStatesBiological，或简称USBiological）位于麻省斯万普斯科特(Swampscott,MA)。作为全美最大的抗原抗体和生化试剂供应商，USBiological目前向全球六十余个国家的科研工作者提供约150,000种抗体、抗原和生化试剂，以质量卓越闻名。主要产品种类包括：抗体、生化试剂、基因克隆相关产品、培养基、生长因子等细胞因子、分子生物学试剂。美国USBiological的产品服务于免疫学，分子生物学，分子遗传学，体外诊断，组织培养等科研领域。USBiological一直恪守“为您的研究减少成本”原则，提供高纯度、高品质的产品，并在大多数生化试剂、生物与培养基产品线上保持最高的性价比。USBiological的产品被广泛用于各种科学研究领域，包括基因组研究、生物技术、药物研发和疾病诊断等。USBiological的产品从小型科研级到生物制品大规模级规格均有提供。众多全球顶ji的生物制药和生物技术公司都在使用USBiological的高品质产品。

usbio的生化试剂，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。

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