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Everest Biotech/L-Lactate Assay Kit I(5ml) 100 Assays- Assay Solution ONLY/120001100A/(5ml)100 assays- ASSAY SOLUTION ON
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Everest Biotech/L-Lactate Assay Kit I(5ml) 100 Assays- Assay Solution ONLY/120001100A/(5ml)100 assays- ASSAY SOLUTION ON
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Everest Biotech
货号 / 
120001100A
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4000-520-616

SampleTyple

Plasma,Serum,Cellculturesupernatant,otherbodyfluid

Contents

Method

Colorimetricmethodat490nm

StandardCurve

Sensitivity

60µM-3000µM

ReactionTime

30minutes

Applications

ForBIOLOGicalresearch:
L-Lactatemeasurementinbiologicalsamples
Fordrug/pharmresearch:
DruginfluenceonL-Lactatemetabolism

Storage

-80°C

Notes

Materialsneededbutnotsupplied

Aplatereadercapableofmeasuringabsorbancebetween470-490nm

AdjustablePipettesandarepeatpipettor

Asourceofpurewater;glassdistilledwaterofHPLC-gradewaterissufficient

0.5MAceticAcid

Clearflatbottom96-wellplatesifnotincludedinthekitpurchased

ShippingIcepacks

ReagentPreparation

Note:Allreagentsarefrozen.SWerecommendyouspinsmallvialsbeforeopening.

1.L-LactateStandards
Thevialcontains1000µlof3mML-LactateStandard.Thestandardmustbeequilibratedtoroomtemperaturebeforeuse.1mlofthestandardisenoughformaking3standardcurvesifassayedinduplicate.Storeat-80?C.


2.L-LactateAssaySolution
Thesolutioncontainsenzymesthatarelightsensitive.Thesolutionmustbethawedonicebeforeuse.Besttoaliquottheamountneededanduseitalltopreventthawing/freezingcycles.Freezeandstorealiquotsat-80oC

Protocol

1.SamplePreparation

Serum/Plasma/otherbodyfluid/cellculturesupernatant
Serum,Plasma,otherbodyfluid,orcellculturesupernatantcanbemeasureddirectlybyaseriesofdilutionsofthesample(1/2;1/4;1/8;.…..)toensurethereADIngsarewithinthestandardcurve.
YoursamplescanbedilutedwithdH2O.
Note:ifsamples(likehemolyzedserum/plasmaorcellculturemediumwhichcontainFBS)containhighleveloflactatedehydrogenasecapableofconvertinglactatetopyruvate,itisimportantforthesamplestobedeproteinated.

SolidSamples
Solidsamples,suchastissues,canbefirsthomogenizedandextractedwithethanol(80%)withatissues/Ethanolratioof1:8(1hrat4°C)followedbycentrifugationat10,000xg.Theclearsupernatantsthencanbemeasureddirectlybyaseriesofdilutionsofthesample(1/2;1/4;1/8;.…..)toensurethereadingsarewithinthestandardcurve.YoursamplescanbedilutedwithdH2O.

AddingSamples
Add50μlofsamplestoeachwell.
Werecommendthatsamplesbeassayedinduplicate.

Note:
Ifpreparedsamplesarenotassayedthesameday,storethesamplesat-80?C.Ifsamplesneedtobedeproteinated,makesuretodeproteinizethesamplespriortostoringinthefreezer.Thedeproteinatedsampleswillbestableforonemonthwhilestoredat-80?C.Forfrozensamples,dilutionsofsamplesmustbedonerightbeforeassaying.

2.StandardCurvePreparation
WerecommendthatL-LactateStandardsbeassayedinduplicate.Astandardcurvehastoberunineachassay.
Add50µl,40µl,30µl,20µl,10µl,5µl,1µl,and0µlofL-lactateStandardtoeachwell.Thenadjustvolumeto50μl/wellwithdH2O.

3.Performtheassay

a)Add50ulofL-LactateassaysolutiontoeachwellcontainingL-Lactatestandardsandtestsamples.
b)Incubatefor30minsat37°Cincubator.Note:PleasedonotuseCO2incubator.
c)Stopthereactionbyadding50μlof0.5Maceticacidperwellfollowedbybriefgentleagitation.Note:Eliminateanyairbubblespresentinthewellsusinganeedlepriortomeasurement.
d)Measuretheabsorbanceat490nMusingamicroplatereader.

4.Calculations

a)AveragetheOD490nmvaluesofreplicatewellsofeachL-Lactatestandard,testsamples,andblank.Inordertogetthecorrectedabsorbance,subtracttheaverageOD490nmvalueoftheblank(L-LactateStandard#8)fromtheaverageOD490nmvaluesfromallstandardsandsamples.

b)MakeastandardcurvebyplottingOD490nmvaluesfromeachL-LactatestandardsasafunctionofL-Lactateconcentration.Thiscanbedonewithexcelspreadsheet.CalculatethevalueofL-Lactateinsamplesusingtheequationobtainedfromthelinearregressionofthestandardcurve.

L-Lactate(µM)=[(Correctedabsorbance)-(y-intercept)]/slope

MaterialSafetyDataSheet

Date:Oct26,2015

1.PRODUCTANDCOMPANYIDENTIFICATION

1.1Productidentifier

Productname:L-LactateAssayKitI

CatalogNo:1200011002,120001100A,120001100P,1200012002,120001200A,12001200P,1200014002,120001400A,120001400P

1.2Relevantidentifiedusesofthesubstanceormixtureandusesadvisedagainst

Forresearchuseonly.

1.3Detailsofthesupplierofthesafetydatasheet

Company:EtonBioscienceInc

TollFree:1-800-758-1630

Tel:1-800-758-1630

Fax:1-800-507-2912

1.4Emergencytelephonenumber

1-800-758-1630

2.HAZARDSIDENTIFICATION

2.1Classificationofthesubstanceormixture

Notahazardoussubstanceormixture

2.2GHSLabelelements,includingprecautionarystatements

Notahazardoussubstanceormixture

2.3Otherhazards

None

3.COMPOSITION/INFORMATIONONINGREDIENTS

3.1Substances

4.FIRSTAIDMEASURES

4.1Descriptionoffirstaidmeasures

Eyecontact

Checkforandremovecontactlensesandflushwithcopiousamountsofwater;assureadequateflushingbyseparatingtheeyelidswithfingers;callaphysician

SkinContact

Flushwithcopiousamountsofwater;removecontaminatedclothingandshoes;callaphysician

Inhalation

Removetofreshair.Ifnotbreathinggiveartificialrespiration;callaphysician

Ingestion

Ifswallowed,nevergiveanythingbymouthtoanunconsciousperson.Rinsemouthwithwater;callaphysician

4.2Mostimportantsymptomsandeffects,bothacuteanddelayed

Noinformationavailable

4.3Indicationofanyimmediatemedicalattentionandspecialtreatmentneeded.

Noinformationavailable

5.FIREFIGHTINGMEASURES

5.1Extinguishingmedia

Suitableextinguishingmedia

Waterspray,alcohol-resistantfoam,drychemicalorcarbondioxide

5.2Specialhazardsarisingfromthesubstanceormixture

Noinformationavailable

5.3Adviceforfirefighters

Wearself-containedbreathingapparatusandprotectiveclothingtopreventcontactwithskinandeyes.

6.ACCIDENTALRELEASEMEASURES

6.1Personalprecautions,protectiveequipmentandemergencyprocedures

Usefullpersonalprotectiveequipment.Evacuatepersonneltosafeareas.Avoidbreathingvapors,mistorgas.

6.2Environmentalprecautions

Preventfurtherleakageorspillage.Preventproductfromenteringdrain.

6.3Methodsandmaterialforcontainmentandcleaningup

Keepinsuitable,closedcontainersfordisposal

7.HANDLINGANDSTORAGE

7.1Precautionsforsafehandling

Avoidskin/eyecontact.Useprotectiveequipmentasneeded.Washcontaminatedclothingbeforereuse.

7.2Conditionsforsafestorage,includinganyincompatibilities

Keepcontainertightlyclosed.Storeat-80?C.

7.3Specificenduse(s)

Nodataavailable.

8.EXPOSURECONTROLS/PERSONALINFORMATION

8.1ControlParameters

Thisproductcontainsnohazardousmaterialswithoccupationalexposurelimits.

8.2Exposurecontrols

Engineeringcontrols

Provideshowerandeyewashstation

Personalprotectiveequipment

L-lactateAssaySolution

Components

CAS#

DMSO

67-68-5

L-LDH

9001-60-9

Tris-HCl,pH7.5

N/A

Water

7732-18-5

L-LactateAssayStandard

Components

CAS#

SodiumL-Lactate

867-56-1

Tris-HCl,pH7.5

N/A

Water

7732-18-5









EyeProtection

Wearsafetygoggles

SkinProtection

Wearprotectiveclothing

HandProtection

Wearprotectivegloves

Respiratoryprotection

WearNIOSH/MSHAapprovedrespirators

9.PHYSICALANDCHEMICALPROPERTIES

9.1Informationonbasicphysicalandchemicalproperties

L-LactateAssaySolution

Appearance

Liquid,yellowish

pH

7.5

Meltingpoint

Nodataavailable

Freezingpoint

Nodataavailable

Boilingpoint

Nodataavailable

Density

Nodataavailable

Watersolubility

Soluble

L-LactateStandard

Appearance

Liquid,colorless

pH

7.5

Meltingpoint

Nodataavailable

Freezingpoint

Nodataavailable

Boilingpoint

Nodataavailable

Density

Nodataavailable

Watersolubility

Soluble

9.2Othersafetyinformation

Nodataavailable.

10.STABILITYANDREACTIVITY

10.1Reactivity

Nodataavailable

10.2Chemicalstability

Stableunderrecommendedstorageconditions.

10.3Possibilityofhazardousreactions

Nodataavailable.

10.4Conditionstoavoid

Nodataavailable

10.5IncompatIBLematerials

Strongoxidizingagents

10.6Hazardousdecompositionproducts

Hazardousdecompositionproductsformedunderfireconditions:carbonoxides

11.TOXICOLOGICALINFORMATION

11.1Informationontoxicologicaleffects

Toxicologicaleffectsonthisproducthavenotbeenthoroughlystudied.

12.ECOLOGICALINFORMATION

12.1Toxicity

Avoidreleaseintoenvironment

12.2Persistenceanddegradability

Nodataavailable

12.3.Bioaccumulativepotential

Nodataavailable

12.4Mobilityinsoil

Nodataavailable

12.5ResultsofPBTandvPvBassessment

Nodataavailable

12.6Otheradverseeffects

Nodataavailable

13.DISPOSALINFORMATION

13.1Wastetreatmentmethods

Disposeinaccordancewithlocal,state,andfederalregulations.

14.TRANSPORTINFORMATION

DOT:

Propershippingname:none
Non-Hazardousfortransport:thissubstanceisconsideredtobenon-hazardousfortransport
IATA:

Propershippingname:none
Non-Hazardousfortransport:thissubstanceisconsideredtobenon-hazardousfortransport

ADR/RID

Propershippingname:none
Non-Hazardousfortransport:thissubstanceisconsideredtobenon-hazardousfortransport

15.REGULATORYINFORMATION

EURiskandSafetyphrases:

R36/37/38:irritatingtoeyes/respiratorysystem/skin

16.OTHERINFORMATION

Theinformationaboveisbelievedtobeaccurateandrepresentsthebestinformationcurrentlyavailabletous.However,wemakenowarrantyofmerchantabilityoranyotherwarranty,expressorimplied,withrespecttosuchinformation.EtonBioscienceInc.shallnotbeheldliableforanydamagesorotherconsequencesresultingfromhandlingorfromcontactwiththeaboveproduct.


Q.WhattypeofmediumshouldIuseformakingculturedcellsforLactateassay?
A.Pleaseusephenolredfreemedium.Pleasedonotusephenolredmediumsincephenolredwouldaffectabsorbancereadings.

Q.WhatenzymesisusedinLactateAssay?
A.ItisLDH.

Q.CanyoutellmethecompositionsofyourLactateAssay?
A.Unfortunatelyitisproprietary.


Q.HowdoIknowwhetherIneedtouseL-LactateorD-Lactateassaykit?
A.L-Lactateassaykitsarecommonlyusedformeasuringlactatelevelinanimalcells.
D-lactateassaykitsarecommonlyusedformeasuringlactatelevelinbacteriacells.

Q.DoIneedtomakeastandardcurveeverytime?
A.Yes,itisnecessarysinceyoucalculateyoursampleconcentrationbasedonthestandardcurve.PleasedonotusetherepresentativeLactatestandardcurveintheprotocoltocalculateconcentrationsofyoursamples.

Q.CanIusefluorescencespectroscopytomeasurereadingsforLactateassaykits?
A.No,thekitdoesnotworkwithafluorescencereadersincethekitemployscolorimetricassays.

Q.HowdoImeasureLactatefromculturedcells?
A.Youcangrowcellsinphenolred-freemedium,thenyoucanextractcellswith80%ethanol.

Q.CanIusefrozensamples?
A.Althoughitisbettertousefreshsamplesforassays,youcanusefrozensamples.Ifyouarenotusingfrozensamplesinonce,pleasemakealiquotsofyoursamplesbeforeyouputtheminafreezertopreventfromdegradingsamplesfromrepeatedfreeze-thawcycles.
Q.Mysampleformedpreciptationafteraddingaceticacid.HowcanIpreventthis?
A.Youcanstillmeasurereadingswithoutaddingaceticacid.
Q.Theprotocolrecommendsmeasuringtheabsorbanceat490nm.Is485nmcloseenough?
A.Itisokaslongasyousetuptheabsorbancebetween470and490nm.
Q.WillL-LactateassaymeasureD-Lactate?
A.No,itwont.
Q.CanIuseatransparentwall96-wellplatewithLactateKit?
A.Yes,youcan.

Q.CanEthanolinterferewiththeenzyme?

A.Theenzymecantolerateupto20%ofethanolwithoutaffectingit’senzymaticactivitywithL-Lactate.

SamVandeVelde,MeghanF.Hogan,andMarcMontminy.:mTORlinksincretinsignalingtoHIFinductioninpancreaticbetacells.PNAS,Oct2011;108:16876-16882.

JunMifune,KatrinGrage,andBerndH.A.Rehm.:ProductionofFunctionalizedBiopolyesterGranulesbyRecombinantLactococcuslactis.Appl.Envir.Microbiol.,Jul2009;75:4668-4675.

DragutinJ.Savic,WilliamM.McShan,andWilliamM.McShan.:Long-termsurvivalofStreptococcuspyogenesinrichmediaispH-dependent.Microbiology,Jun2012;158:1428-1436

KristinA.Anderson,FuminLin,ThomasJ.Ribar,RobertD.Stevens,MichaelJ.Muehlbauer,ChristopherB.Newgard,andAnthonyR.Means.:DeletionofCaMKK2fromtheLiverLowersBloodGlucoseandImprovesWhole-BodyGlucoseToleranceintheMouse.Mol.Endocrinol.,Feb2012;26:281-291.

JaninaP.Lewis,DivyaIyer,andCeciliaAnaya-Bergman.:AdaptationofPorphyromonasgingivalistomicroaerophilicconditionsinvolvesincreasedconsumptionofformateandreducedutilizationoflactate.Microbiology,Nov2009;155:3758-3774.

AmparoWolf,SameerAgnihotri,JohannMicallef,JoydeepMukherjee,NesrinSabha,RobCairns,CynthiaHawkins,andAbhijitGuha.:Hexokinase2isakeymediatorofaerobicglycolysisandpromotestumorgrowthinhumanglioblastomamultiforme.J.Exp.Med.,Feb2011;208:313-326.

InnaSerganova,AsifRizwan,XiaohuiNi,SunithaB.Thakur,JelenaVider,JamesRussell,RonaldBlasberg,andJasonA.Koutcher.:MetabolicImaging:ALinkbetweenLactateDehydrogenaseA,Lactate,andTumorPhenotype.Clin.CancerRes.,Oct2011;17:6250-6261.

ChangluLiu,JiejunWu,JessicaZhu,ChesterKuei,JingxueYu,JonathanShelton,StevenW.Sutton,XiaorongLi,SuJinYun,TaranehMirzadegan,CurtMazur,FredrikKamme,andTimothyW.Lovenberg.:METABOLISM,REGULATION,ANDSIGNALING:LactateInhibitsLipolysisinFatCellsthroughActivationofanOrphanG-protein-coupledReceptor,GPR81.J.Biol.Chem.,Jan2009;284:2811-2822.

MarinaOstroukhova,NicholasGoplen,MdZunayetKarim,LidiaMichalec,LeiGuo,QiaolingLiang,andRafeulAlam.:Theroleoflow-levellactateproductioninairwayinflammationinasthma.AmJPhysiolLungCellMolPhysiol,Feb2012;302:L300-L307.
Zhuang,Yongxian,DanielKChan,andWKeithMiskimins.“PreventingFeedbackActivationofGlycolyticATPProductionEnhancesMetforminCytotoxicityinBreastCancerCellsWhenOxidativePhosphorylationIsInhibited.”Cancer&Metabolism2.Suppl1(2014):P89.PMC.Web.6Oct.2015.
Lezi,E.etal.“EffectofExerciseonMouseLiverandBrainBioenergeticInfrastructures.”Experimentalphysiology98.1(2013):207–219.PMC.Web.6Oct.2015.
YongxianZhuang,DanielK.Chan,AllisonB.Haugrud,W.KeithMiskimins.MechanismsbyWhichLowGlucoseEnhancestheCytotoxicityofMetformintoCancerCellsBothInVitroandInVivo.September25,2014DOI:10.1371/journal.pone.0108444

BorgesFT1,MeloSA,ÖzdemirBC,KatoN,RevueltaI,MillerCA,GattoneVH2nd,LeBleuVS,KalluriR.TGF-β1-containingexosomesfrominjuredepithelialcellsactivatefibroblaststoinitiatetissueregenerativeresponsesandfibrosis.JAmSocNephrol.2013Feb;24(3):385-92.doi:10.1681/ASN.2012101031.Epub2012Dec28.

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百奥森18分钟霉菌毒素检测试剂盒,有需要的可以了解下!多种规格型号可供选择

产品名称

黄曲霉毒素B1(AFB1)酶免检测试剂盒

黄曲霉毒素B1(AFB1)酶免检测试剂盒

赭曲霉毒素A(OTA)酶免检测试剂盒

玉米赤霉烯酮(ZEN)酶免检测试剂盒

玉米赤霉烯酮(ZEN)酶免检测试剂盒

呕吐毒素(DON)酶免检测试剂盒

黄曲霉毒素M1(AFM1)酶免检测试剂盒

总黄曲霉毒素(AFT)酶免检测试剂盒

T-2毒素(T-2)酶免检测试剂盒

伏马毒素B1(FB1)酶免检测试剂盒


二楼说的太对了,我以前也用亚能的试剂做实验,每次都要过夜,太TM烦了,后来遇到港龙生物的来推销,就换了。

1、请问我用NEB的PNGaseF处理糖蛋白后,上样跑电泳之前需不需要再煮沸一次蛋白?因为第一步加入糖蛋白变性缓冲液后会有一个煮沸变性的步骤,不知加入PNGaseF后还用再煮沸一遍吗?

2、如果经过脱糖处理后的蛋白想继续下一步实验用的话,怎样把它纯化出来?透析还是超滤?之前试过超滤除盐,损失太大。

3、另外各位有没有用过的检测糖蛋白含不含有糖基的试剂盒推荐?简单检测“有或无”的那种就可以,品牌能提供一下最好啦!

谢谢!

你购买的是血液的还是唾液的,但艾滋病还是要早发现,早预防。如果没有身份证,建议去医院检查,挂皮肤科,给医生说检查艾滋,就可以了。如果爱面子,京东山 爱/卫自己测。如果有身份证,那就带上,给CDC人员说检查艾滋,他们会问你用什么方法,有快速法和酶免法,快速法10分钟拿结果,酶免法1至7天拿结果,但不管什么方法,都是很准确的。希望能帮到你。
H7N9禽流感病毒核酸检测试剂盒123
瓴泶瑿寕胲Qck82018-02-02
试剂盒,是指能完成一个特定实验的必需的试剂/器材的集合.试剂盒具有简单、快速、方便,适于现场操作等特点,对基体分析操作技能要求降低,更易实现流水化、标准化管理,有效排除了实验人员主观因素的影响,降低实验过程中的偶然误差,被广泛应用于检测领域.
试剂盒在全球市场上的研发与销售呈快速上升趋势,2005年全球市场销售额超过200亿美元,且以15%左右的速度逐年增长.一方面是试剂盒的迅猛发展,而另一方面试剂盒市场良莠不齐的现象愈加明显,试剂盒的生产、销售及认证认可体制尚不完善,没有相应的标准或质量评价政策.且其灵敏度,稳定性及假阴/阳性控制尚不能满足检测需要,采用试剂盒进行检测的公信度受到质疑.
同时,食品安全领域是当前问题最多、最受关注的领域,这个领域的检测包括了物理、化学、微生物及分子生物学基础理论,无论是按检测原理、用途还是其它分类方式,涉及食品安全检测项目的试剂盒的品种是最全最多的.因此,从该领域着手从事评价制度的研究,便于获得基础性数据结果,并由此推广至动植物检疫及其它领域.
随着H7N9禽流感疫情的不断发散,国家流感中心已经多次发放人感染H7N9禽流感检测试剂,覆盖了全国31个流感网络实验室,并表示,诊断试剂的广泛发放是实现关口前移,控制疫情传播、蔓延的重要手段.而一旦H7N9监测关口继续前移,主动监测范围扩大,病毒检测试剂的需求量将进一步加大.
可采用H7N9亚型禽流感病毒RNA检测试剂盒(荧光PCR法)和H7N9禽流感病毒核酸检测试剂盒(PCR-荧光探针法),定价分别为48人份/盒和48反应/盒,相比市场此前预期的100-200元之间的价格定位低了很多.在检验方法上,卫纪委提醒,前者需要配备全自动荧光PCR检测仪专用PCR扩增管和核酸分离试剂盒(硅胶膜吸附法)等必须设备及咽拭子样本,后者卫计委推荐采用达安基因生产的核酸提取试剂盒进行检验.

我用Promega公司的双荧光素酶检测试剂盒(E2920)检测到的firefly萤光素酶活性很低,只有4*10的3次方;海肾萤光素酶活性有10的6次方。

我用Ad293细胞做了转染,fireflyluciferase质粒:RanillaLuciferase质粒=0.1ug:0.025ug/一个孔(96孔板),共转染了二天,再进行双萤光素酶检测。

我想了解fireflyluciferase活性用promega的这个E2920-双萤光素酶试剂盒检测得到10的3次方,这种数值正常吗?

我做了3次重复实验,每次firefly萤光素酶活性很低,只有4*10的3次方;而海肾萤光素酶活性有10的6次方。

求指教?fireflyluciferase活性低?会是什么原因呢?

请问有园友用过碧云天的抗酒石酸酸性磷酸酶检测试剂盒吗?求交流,感谢!
ldh试剂盒与ctl毒性杀伤检测试剂盒一样的
ldh试剂盒指乳酸脱氢酶检测试剂盒,是一种基于diaphorase催化的INT显色反应,通过比色法检测细胞毒性时释放的乳酸脱氢酶活性或检测其它样品中的乳酸脱氢酶活性的试剂盒。可以用于常规的乳酸脱氢酶活性的检测,更常用于以LDH释放为指标的细胞毒性检测。
ctl毒性杀伤检测试剂盒是基于LDH在胞浆内含量丰富,正常时不能通过细胞膜,当细胞受损伤或死亡时可释放到细胞外,此时细胞培养液中LDH活性与细胞死亡数目成正比,用比色法测定并与靶细胞对照孔LDH活性比较,可计算效应细胞对靶细胞的杀伤。

G6PD酶活性检测问题:

G6PDHactivityreportedasnmole/min/mL(milliunit/mL):Oneunitistheamountofenzymethatcatalyzestheconversionof1.0µmoleofglucose-6-phosphateto6-phosphoglucono-δ-lactoneandgenerates1.0µmoleofNADHperminuteat37°C.

Sigma和BioVision的G6PD活性试剂盒都是检测生成的NADH,请问各位老师,为什么是NADH而不是NADPH呢,此为何解?

那位大侠可以帮助小弟,需要检测线粒体呼吸链复合物活性,想找相关试剂盒,找到上海杰美有,不知道有谁用过,效果怎么样,还有没有别的好的推荐。
Insulin牛胰岛素试剂盒123
傲爆头1592018-02-06
牛胰岛素:自牛胰腺提取而来,分子结构有三个氨基酸与人胰岛素不同,疗效稍差,容易发生过敏或胰岛素抵抗。动物胰岛素唯一的优点就是价格便宜。患者可以轻松负担。

牛胰岛素,是一种多肽

在1965年9月17日我国完成了结晶牛胰岛素的全合成。经过严格鉴定,它的结构、生物活力、物理化学性质、结晶形状都和天然的牛胰岛素完全一样。这是世界上第一个人工合成的蛋白质,为人类认识生命、揭开生命奥秘迈出了可喜的一大步。这项成果获1982年中国自然科学一等奖。
1953年,英国人F. SangerSanger由于测定了牛胰岛素的一级结构而获得1958年诺贝尔化学奖。
1.原子标记技术Eu离子通过双功能螯合剂与被标记物相连,Eu螯合剂分子量仅600左右。因此对被标记的物质影响非常最小。用生物素、酶等大分子标记物,须通过一系列化学反应来检测,其过程受影响因素较多,用......