Description:
TheTECO®SerumCollagenIVELISAprovidesamethodforthequantitativedeterminationofcollagenIVinhumanserum.
KitComposition:
- CollagenIVAntibodycoatedMicroassayPlate12stripsof8wells(96breakapartwellsintotal),inaframe.ReadytoUse.
- CollagenIVCalibratorStockSolution,1000ug/L
- 10XWashBufferConcentrate
- DilutionBufferReadytoUse
- EnzymeConjugate:Anti-collagenIVmouseFab’conjugatedtoHRP,ReadytoUse
- TMBSubstrateReadytoUse
- StopSolution1MSulphuricAcid,ReadytoUse
- UncoatedMicroassayPlateReadytoUse
AssayPrinciple:
TheTECO®SerumCollagenIVELISAisdesignedfortheassayofserumcollagenIV.Itisasolidphaseone-stepsandwichELISA.CollagenIVinthesampleisboundsimultaneouslybyasolid-phasemonoclonalantibodyandamonoclonalantibody-enzymeconjugate,eachdirectedatdifferentantigenicsites.ThisresultsinthecollagenIVmoleculebeingsandwichedbetweenthesolidphaseandenzyme-labelledantibodies.Afterremovingunboundenzyme-labelledantibodyandsample,theplateisincubatedwithenzymesubstrate.TheresultantcolordevelopmentisdirectlyproportionaltotheamountofcollagenIVinthesample.
TypicalCalibrationCurve
TypicalcalibrationcurveobtainedusingSerumCollagenIVELISA.4-ParameterplotofA450/630nmversusug/LCollagenIV.Assayrangeis15.6-1000ug/LCollagenIV.
Background:
CollagenIVisatypeofnon-helicalformingcollagenfoundprimarilyisthebasallamina(basementmembrane).Chronicliverdiseasecomprisesanumberofprogressivedisorderswhichculminateinlivercirrhosisandwhicharecharacterizedbyexcessivedepositionofcollagen.
Althoughvarioustypesofcollagen(typeI,III,IV,VandVI)increaseintheliverwiththeprogressionoffibrosis,typeIVcollagen,aconstituentofthebasementmembrane,isparticularlynoteworthyforthefollowingreasons:itsserumlevelcorrelateswithhepaticlevelsofcollagenIV,serumlevelsofcollagenIVfallinresponsetoeffectivetherapy,anditistheearliesttypeofcollagentobesynthesizedduringexperimentalliverinjury.SerumcollagenIVlevelsareelevatedinavarietyofliverdiseases,andhavebeenfoundtobepredictiveoftherapyresponseinHepatitisCinfectionandtobesensitiveindicatorsoftherapyresponseinabstainingalcoholics.
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请问下有无同学需要H37RA的？我是做EAE模型的，上个月购买了BDDifco公司的H37RA（货号），因为购买的时候只能整盒6支购买，但我们用不了那么多，所以想问问有无同学需要的，100mg/支，800元/支或用等价试剂交换。地址广州。有需要的请私信，谢谢！
A、Na2CO3HClH2SO4NaNO3
B、K2CO3H2SO4HNO3BaCl2
C、HClHNO3AgNO3NaCl
D、NaOHFeCl3MgCl2BaCl2
我想检测血管组织中的钙离子浓度，不知道哪个公司有试剂盒
2.NaHCO3,Ba(OH)3,H2SO4
3.HCL,NaAlO2,NaHSO4
4.Ca(OH)2,Na2CO3,BaCO3
谢谢了
要原因
bhclna2so4nano3na2co3
chclnaohna2co3nacl
dba(oh)2nahco3alcl3nahso4
C.MgCl2溶液和氨水D.盐酸和NaAlO2溶液
为什么
