Description:
TheAR-chipwasspecificallydesignedforthequantitativeanalysisofthewhitethrombusformationprocessmediatedbytheactivationofbothplateletsandcoagulationsystemunderflowconditions.Developmentofwhitethrombiconsistingofactivatedplateletsandfibrincanbeanalyzedandvisualizedbymeasuringflowpressurewaveformsandvideo-microscopicinspection,respectively.
Imageanalysisofwhitethrombiformation
fibrinrichplateletthrombusformation
fibrinrichplateletthrombusformation(GPIbantagoNIST)
Characteristics:
CharacteristicsoftheAR-chipassay
- Usesatissuethromboplastin-andcollagen-coatedmicrochip
- Requiresonlyasmallamountofwholeblood(450μl)
- Simplesettingofoptionalwallshearrate(200-800s-1）
- Dual-monitoringsystem(byflowpressurewaveformandreal-timevideoimaging)
- Easyoperationcontrolledbythecomputerinterlockingsystem
MeasurementPrinciple:
ArecalicifiedbloodsamplecontainingCTI(corntrypsininhibitor)isplacedinareservoirthatisconnectedtoahigh-precisionmicropump.Themicropumpinfusesmineraloilintothereservoir,whichimpelsthesamplebloodthroughtheinletportandintotheflowpaththatleadstothetissuethromboplastin-andcollagen-coatedanalyticalpath.
Theprocessofwhitethrombusformation(WTF)intheflowchamberiscontinuouslymonitoredbyavideo-microscope(10x)positionedundertheflowchamberandbyapressuresensorthatrecordspressurechangesintheflowpath.Theflowrateofthemicropumpcanbeeasilyadjustedasneededbasedonthedesiredexperimentalconditions(range:4-40μl/min).
WhenbloodflowsthroughtheanalyticalpathoftheAR-chip,theextrinsiccoagulationpathwayandplateletsaresimultaneouslyactivatedonthetissuethromboplastin-andcollagen-coatedsurfaces.Theactivatedcoagulationsystemandplateletsthenamplifytheirrespsonsesresultinginthrombusgrowth,whichoccludesthecapillaryandincreasestheflowpressure.
Parameters:
Flowpressureanalysis
WhenbloodflowsthroughtheanalyticalpathofAR-chip,theplateletsandcoagulationsystemaresimultaneouslyactivatedonthecollagen-andtissuethromboplastin-coatedsurface.Smallwhitethrombithatconsistofactivatedplateletsandfibrinfibersgraduallyincreaseinsizeandeventuallyoccludethecapillary,resultinginanincreaseofflowpressure.Thus,flowpressurepatternsreflectthewhitethrombusformationprocess.
Definitionsoftheparametersusedforthequantificationofthewhitethrombusformationprocess
Threespecificparameters,T10,T80,andAUC30,areusedtoquantifywhitethrombusformationinsidetheAR-chip.
T10(=timetoreach10kPa)isdefinedastheonsetofwhitethrombusformationandrepresentstheduration(sec)fortheflowpressuretoincreaseto10kPafrombaseline.
T80(=timetoreach80kPa)isdefinedasthecompleteocclusionofcapillaryduetothrombusformationandrepresentstheduration(min)fortheflowpressuretoincreaseto80kPafrombaseline.
AUC30(=areaundertheflowpressurecurvefor30min)isusedtoquantifythrombogenicitywhenT80cannotbeobtainedduetoreducedthrombusformationorfragilethrombi.
Antithromboticagents
AswhitethrombithatdevelopinAR-chipsconsistofactivatedplateletsandfibrinfiberscontainingabundantthrombin,theAR-chipassayiscapableofanalyzingsingleandintegratedefficacies,basedoncommonparameters,ofvariousantithromboticagents,includinganticoagulant,antiplatelet,andthrombolyticagents,undervariableflowconditions.
WhitethrombiformedinanAR-chip
(red,fibrin;green,activatedplatelets)
Imageanalysisofwhitethrombusformation
WhitethrombusformationintheAR-chipcanbeautomaticallymonitoredandrecordedusingabuilt-invideomicroscopeatthree-minutesintervals.
Imagesofwhitethrombiformedinthecapillarycanbeautomaticallyprocessedfromtherecordedvideo,andtheareacoveredwithwhitethrombicanbeidentifiedandcalculatedusingtheZIAprogram.Thus,inadditiontoflowpressureanalysis,thewhitethrombusformationprocesscanbeopticallyanalyzedandquantifiedinAR-chipmeasurements.
AssayProtocol:
PlaceanAR-chipontheassaystageandletstandfor1-3mintoallowstABIlizationofthetemperature.
Connectthereservoirtothenozzle.
Mixacitratedwhole-bloodsamplewiththeCa/CTIreagent,pipet450μlofthemixedsampleintothereservoir,coverwithalid,andthenremovethecap.
ConnectthereservoirtotheAR-chip.
Pushthestartbutton.
ebiomall.com
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2.NaHCO3,Ba(OH)3,H2SO4
3.HCL,NaAlO2,NaHSO4
4.Ca(OH)2,Na2CO3,BaCO3
谢谢了
要原因
（1）优级纯试剂 亦称保证试剂，为一级品，纯度高，杂质极少，主要用于精密分析和科学研究，常以GR表示。
（2）分析纯试剂 亦称分析试剂，为二级品，纯度略低于优级纯，杂质含量略高于优级纯，适用于重要分析和一般性研究工作，常以AR表示。
（3）化学纯试剂 为三级品，纯度较分析纯差，但高于实验试剂，适用于工厂、学校一般性的分析工作，常以CP表示。
（4）实验试剂 为四级品，纯度比化学纯差，但比工业品纯度高，主要用于一般化学实验，不能用于分析工作，常以 LR表示。
以上按试剂纯度的分类法已在我国通用。根据化学工业部颁布的“化学试剂包装及标志”的规定，化学试剂的不同等级分别用各种不同的颜色来标志，见表1。
表1 我国化学试剂的等级及标志
D,K2SO4Na2CO3BaCL2NaNO3
刚入这行，谢谢大家
C.MgCl2溶液和氨水D.盐酸和NaAlO2溶液
为什么
我想检测血管组织中的钙离子浓度，不知道哪个公司有试剂盒
A.H2SO4Na2SO4AgNO3BaCl2.
B.NaOHNa2CO3NaHSO4MgCl2
C.CaCl2NaNO3MgSO4BaCl2
D.HNO3KOHKClK2CO3
bhclna2so4nano3na2co3
chclnaohna2co3nacl
dba(oh)2nahco3alcl3nahso4
