This is a single issue of Methods, a companion journal to Methods in Enzymology, focusing on rapidly developing techniques. This single issue is dedicated to Macromolecular Crystallization and begins with an Introduction to Protein Crystallization by Alexander McPherson.
The issue features the following additional thirteen articles:
•Protein crystallization and phase diagrams, Neer Asherie.
•Growth and disorder of macromolecular crystals: insights from atomic force microscopy and x-ray diffraction studies, Alexander J. Malkin and Robert E. Thorne.
•Ions from the Hofmeister series and osmolytes: effects on proteins in solution and in the crystallization process, Kim D. Collins.
•Effects of naturally occurring osmolytes on protein stability and solubility; issues important in protein crystallization, D.W. Bolen.
•Practical aspects of using the microbatch method in screening conditions for protein crystallization, Allan D’Arcy, Aengus Mac Sweeney, and Alexander Haber.
•Automated systems for protein crystallization, Joel Bard, Kimberly Ercolani, Kristine Svenson, Andrea Olland, and Will Somers.
•Lipidic cubic phases as matrices for membrane protein crystallization, Peter Nollert.
•The use of recombinant and molecular engineering in protein crystallization, Zygmunt Derewenda.
•A pedestrian guide to membrane protein crystallization, Michael Wiener.
•Crystallization data mining in structural genomics: using positive and negative results to optimize protein crystallization screens, Rebecca Page and Raymond C. Stevens.
•Predictive models for protein crystallization, Bernhard Rupp and Junwen Wang.
•Crystallization of RNA and RNA-protein complexes, Ailong Ke and Jennifer A. Doudna.
•Macromolecular cryocrystallography – methods for cooling and mounting protein crystals are cryogenic temperatures, J.W. Pflugrath
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2.NaNO3FeCl3AgNO3
分别有什么现象？谢谢回答！
请问有什么方法检测细胞内活性氧更好，各位有什么经验，及相关试剂盒推荐一下。
我想检测血管组织中的钙离子浓度，不知道哪个公司有试剂盒
D,K2SO4Na2CO3BaCL2NaNO3
（1）优级纯试剂 亦称保证试剂，为一级品，纯度高，杂质极少，主要用于精密分析和科学研究，常以GR表示。
（2）分析纯试剂 亦称分析试剂，为二级品，纯度略低于优级纯，杂质含量略高于优级纯，适用于重要分析和一般性研究工作，常以AR表示。
（3）化学纯试剂 为三级品，纯度较分析纯差，但高于实验试剂，适用于工厂、学校一般性的分析工作，常以CP表示。
（4）实验试剂 为四级品，纯度比化学纯差，但比工业品纯度高，主要用于一般化学实验，不能用于分析工作，常以 LR表示。
以上按试剂纯度的分类法已在我国通用。根据化学工业部颁布的“化学试剂包装及标志”的规定，化学试剂的不同等级分别用各种不同的颜色来标志，见表1。
表1 我国化学试剂的等级及标志
