Highlights
- Simple: Quickly and easily rescue plasmid from yeast.
- Efficient Isolation: Works well with low-copy and hard-to-isolate plasmids.
- High-Quality: Isolated plasmid DNA is ideal for molecular biology techniques, such as PCR, transformation, hybridization, etc.
Description
| Applicable For | Plasmid DNA is well suited for downstream applications such as PCR, transformation, hybridization and other sensitive applications |
|---|---|
| Elution Volume | ≥ 10 µl per well |
| Equipment | Centrifuge with microplate carriers |
| Processing Volume | ≤ 1.5 ml of Culture |
| Sample Source | Cell Culture (Colonies/Patches or Liquid Culture) |
| Size Range | Up to 25 kb. |
| Yield | Typically between 0.01-0.3 ng for most 2 µ based plasmids from 1.5 ml overnight cultures |
Q1: What is the difference between Zymoprep Yeast Plasmid Miniprep I and Miniprep II?
Both the Zymoprep Yeast Plasmid Miniprep I and II utilize the same chemistry for lysis; however, Miniprep I uses isopropanol precipitation and Miniprep II utilizes a column for purification. The Miniprep II allows for consistent yield and purity; and samples can be concentrated to a low elution volume.
Q2: What is the typical plasmid yield?
Typically, between 0.01 - 0.3 ng for most 2 µ based plasmid from 1.5 ml overnight cultures.In order to generate more plasmid, the plasmid is typically transformed into E. Coli, cultured, and isolated using a traditional E. Coli plasmid prep.
Q3: Can this kit be used to isolate linear plasmid DNA?
Yes
Q4: If I’m using stationary phase yeast cells, what can I do to improve sample lysis?
We generally recommend working with fresh or early log phase cells, which are easier to lyse. For stationary phase cells, user optimization is necessary, and we recommend increasing digestion to > 1 hour and/ or increasing the amount of Zymolyase.
Q5: What yeast strains are these kits compatible with?
Any strains susceptible to Zymolyase, which includes the following fungal genera: Asbya KloekeraCandidaKluyveromycesDebaryomycesLipomycesEremotheciumMetschikowiaEndomycesPichiaHansenulaPullulariaHanseniasporaSaccharomycesSaccaromycodesSaccharomycopsisSchizosaccahromycesTorulopsis
| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| D2004-1-45 | Solution 1 Digestion Buffer | 45 ml | $88.00 | |
| D2004-1-90 | Solution 1 Digestion Buffer | 90 ml | $146.00 | |
| D2004-2-45 | Solution 2 Lysis Buffer | 45 ml | $88.00 | |
| D2004-2-90 | Solution 2 Lysis Buffer | 90 ml | $146.00 | |
| D2004-3-180 | Solution 3 Neutralizing Buffer | 180 ml | $146.00 | |
| D2004-3-90 | Solution 3 Neutralizing Buffer | 90 ml | $88.00 | |
| D4003-2-24 | DNA Wash Buffer (Concentrate) | 24 ml | $33.00 | |
| D4003-2-48 | DNA Wash Buffer (Concentrate) | 48 ml | $60.00 | |
| C1001-50 | Collection Tubes | 50 Pack | $15.00 | |
| C2002 | Collection Plate | 2 Plates | $22.00 | |
| C2003 | Elution Plate | 2 Plates | $19.00 | |
| C2004 | Zymo-Spin I-96 Plate | 2 Plates | $142.00 | |
| P1001-2 | 96-Well Block | 2 Blocks | $18.00 | |
| C2007-8 | 96-Well Plate Cover Foil | 8 Foils | $18.00 | |
| C2011-2 | Air Permeable Sealing Cover | 2 Pack | $12.00 | |
| C2011-4 | Air Permeable Sealing Cover | 4 Pack | $24.00 | |
| C2011-8 | Air Permeable Sealing Cover | 8 Pack | $42.00 |
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C.MgCl2溶液和氨水D.盐酸和NaAlO2溶液
为什么
bhclna2so4nano3na2co3
chclnaohna2co3nacl
dba(oh)2nahco3alcl3nahso4
A、Na2CO3HClH2SO4NaNO3
B、K2CO3H2SO4HNO3BaCl2
C、HClHNO3AgNO3NaCl
D、NaOHFeCl3MgCl2BaCl2
我想检测血管组织中的钙离子浓度,不知道哪个公司有试剂盒
(1)优级纯试剂 亦称保证试剂,为一级品,纯度高,杂质极少,主要用于精密分析和科学研究,常以GR表示。
(2)分析纯试剂 亦称分析试剂,为二级品,纯度略低于优级纯,杂质含量略高于优级纯,适用于重要分析和一般性研究工作,常以AR表示。
(3)化学纯试剂 为三级品,纯度较分析纯差,但高于实验试剂,适用于工厂、学校一般性的分析工作,常以CP表示。
(4)实验试剂 为四级品,纯度比化学纯差,但比工业品纯度高,主要用于一般化学实验,不能用于分析工作,常以 LR表示。
以上按试剂纯度的分类法已在我国通用。根据化学工业部颁布的“化学试剂包装及标志”的规定,化学试剂的不同等级分别用各种不同的颜色来标志,见表1。
表1 我国化学试剂的等级及标志
通过解答,教会我,谢谢

