Product Specifications:
PRODUCT#: 107
Description: hCEM-15/HIV-1 vif Capture ELISA.
Storage: Store components as specified at 4°C.
Stability: Please see kit label.
Note: Read instructions and familiarize yourself with kit components before performing assays with this kit.
Component List:
A) Plate holder with solid phase hCEM-15 ligand strips (96 well plate). Store at 4°C.
B) Wash Buffer 10 X, (dilute 1:10 with deion-ized water) before use.
C) Sample Dilution Buffer (Store at 4oC)
D) HIV-1 rvif Standard (2ug/ml, Store at 4°C).
E) Detector Reagent: Murine anti-vif mAb peroxidase (dilute 1:100 before use).
F) Peroxidase Substrate (Store at 4°C)
G) Stop Solution (Store at 4°C)
Bring Diluent Buffer (Component C) to room temperature before use.
Section-1
vif Capture
1. vif detection range: 200ng/test to 2ng/test.Transfer 100ul of Dilution Buffer (Component C) to wells B-H. Using a 200ul pipettor, transfer 100ul of vif reference (Component D) to wells A and B each.
2. Using a new pipette tip mix well B contents by drawing the liquid from the well into the pipette tip and gently returing the liquid to the well 10 times without letting any air bubbles into the well. Remove 100ul of mix from well B and transfer to well C. Using the same tip repeat the mixing procedure as in 2 above from wells C to G (2-fold serial dilution ). Leave well H as a blank.
3. Gently tap the plate holder againt a solid object to dislodge any air bubbles in the wells and leave plate at room temperature for 1 hr.
4. Dump contents of wells and wash wells with 1 x wash buffer (Component B: 1:10 diluted with De-ionized H2O) 4 times. Leave last wash in wells until ready for the next step.
Section-2
vif Detection
1. Dilute detector reagent (Component E) 1:100 in Diluent Buffer (Component C). Dump contents of wells (step 4 above) and pipette 100µl of diluted detector reagent (Component E) into each well. Cover plate and leave in a dark place at room temperature for 1 hour.
2. Dump contents of wells, and wash wells 4 times with 1 x wash buffer (Component B). Dump last wash before continuing to the next step.
3. Add 100µl TMB substrate (Component F) to each well.
Note: The TMB Substrate is light sensitive. Do not expose to sustained, direct light.
4. Blue color will develop in Positive Reference wells over 10 minutes at room temperature.
5. Stop color development after10 minutes by adding 50µl of Stop Solution (Component G) to each well. Color will change to yellow. Read plate at 450nm as soon as possible (no more than 10 minutes).
Section-3
Inhibitor Assay
Before adding vif reference to the wells Section 1 above, , add 10 ul of the inhibitor test compound to the wells so that after the addition of 100ul of vif reference to the well, the concentration of the inhibitor in the desired range ( pM, nM, uM and mM) is achieved. Inhibitor test studies may be performed in duplicate or triplicate with appropriate amounts of vif (Section 1 above) to produce the color signal measured at 450nm for readings in 1.5-1.8 OD range. This corresponds to a vif concentration of approximately 100ng/well.
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我想检测血管组织中的钙离子浓度，不知道哪个公司有试剂盒
D,K2SO4Na2CO3BaCL2NaNO3
通过解答，教会我，谢谢
（1）优级纯试剂 亦称保证试剂，为一级品，纯度高，杂质极少，主要用于精密分析和科学研究，常以GR表示。
（2）分析纯试剂 亦称分析试剂，为二级品，纯度略低于优级纯，杂质含量略高于优级纯，适用于重要分析和一般性研究工作，常以AR表示。
（3）化学纯试剂 为三级品，纯度较分析纯差，但高于实验试剂，适用于工厂、学校一般性的分析工作，常以CP表示。
（4）实验试剂 为四级品，纯度比化学纯差，但比工业品纯度高，主要用于一般化学实验，不能用于分析工作，常以 LR表示。
以上按试剂纯度的分类法已在我国通用。根据化学工业部颁布的“化学试剂包装及标志”的规定，化学试剂的不同等级分别用各种不同的颜色来标志，见表1。
表1 我国化学试剂的等级及标志
2.NaNO3FeCl3AgNO3
分别有什么现象？谢谢回答！
C.MgCl2溶液和氨水D.盐酸和NaAlO2溶液
为什么
