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ImmunoTest™ HIV-1 p24 For Serum, Plasma, Tissue Culture Medium ImmunoDX Strictly for non-human research and diagnostics Catalog# IT-200 Intended Use: ImmunoTest™ HIV-1p24 test is an in vitro, semiquantitative immunochromatographic assay for the detection of p24 core protein in blood, serum/plasma, and tissue culture medium samples. Principle: ImmunoTest™ One-Step HIV-1 p24 Test utilizes antibodies specific for the most conserved epitopes of HIV-1 core protein p24 (proprietary) in immunochromatographic assay technology for semiqualitative detection of p24 antigen in, serum, plasma or tissue culture medium. In the test procedure, 20ul of serum/ plasma or tissue culture medium is applied to the sample well (S) of a test cassette. p24 core protein binds to anti-p24 mAb gold conjugate and is then captured by the solid phase p24 specific mAb stripe on the test membrane. Visualization of the p24 core protein in the Test window will occur only when the antibody-dye conjugate binds to the antigen. As the antibody-dye conjugate continues to move along the test membrane, it will bind to another band located in the Control window (C) to generate a colored band regardless of the presence of p24 in the test sample. Therefore, the presence of two colored bands, one in the Test window and the other in the Control window, indicates the presence of p24 core protein in the test sample, while the absence of a distinct colored band in the Test window indicates a negative result. Test Procedure: 1. Tissue culture virus 25ul + 0.05% Tween-20, heat inactivate at 55C for 5 minutes. 2. Transfer 20ul of inactivated test sample (1), or human serum/plasma to sample well (S). 3. Directly deposit 3 drops of developer from the dropper bottle to the sample port (S) in the cassette. 4. Read results in 5 - 30 minutes. Positive: Two colored lines in the Reading windows – one each in the upper Control area and lower Test area indicate the presence of p24 core protein in the test sample. The test result can be read as soon as a distinctive pinkpurple line appears in the Test area. In most strong positive cases, the Test line will appear before the Control line. The Test line may appear after the Control line in some weak positive cases, and the Control line may become darker than the Test line. The three possible positive cases, therefore, are: a. Two strong colored lines, one in each area - T and C . b. One strong colored line in the Test area and one lighter colored line in the Control area. c. One light colored line in the Test area and one strong colored line in the Control area. Negative: Only one colored line in the Control area, with no distinctive colored line in the Test area indicates that no p24 core protein was detected. Invalid: A distinctive colored line should always appear in the Control area. The test is invalid if no line forms in the Control area. Kit Performance: ImmunoTest™ HIV-1 p24 core protein test is a highly specific and sensitive method for the detection of p24 core protein in serum/plasma and tissue culture medium. The test is sensitive to approximately 10pg/ml p24 (0.2pg/test) in the test sample. Stability and Storage: ImmunoTest™ HIV-1 p24 core protein test kit should be stored at ambient temperatures (20- 28 C) in the sealed pouch. The test kit is stable until the expiration date stamped on the pouch when stored under these conditions. Do not use the test if the pouch is damaged or the seal is broken. Keep the test in the sealed pouch until ready for use. Performance Characteristics: In commercially available HIV-1lysates (1mg/ml protein) p24 was detected at 1:100,000 dilution. Recombinant p24 was detected in <0.5pg/20ul range, comparable to the sensitivity of p24 detection by Elisa. p24 antigen positive serum conversion panel members (BBI panels) showed p24 antigen positive tests – distinct T-line, results comparable to Abbotts and Coulters FDA approved p24 Elisa test results. These results were consistent with PCR/RT-PCR test results of these conversion panels. p24 Reference: Using a micro-pipettor, deliver 20ul of the developer to the bottom of the p24 reference vial. Vortex gently and quantitatively transfer the mixed p24 reference to cassette sample (S) port, followed by 2 drops of the developer into the sample port (S). Positive red T-line will appear in a few minutes which will enhance significantly in 10 minutes. p24 reference positive red T-line validates p24 human serum test results. |
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我想检测血管组织中的钙离子浓度，不知道哪个公司有试剂盒
D,K2SO4Na2CO3BaCL2NaNO3
通过解答，教会我，谢谢
（1）优级纯试剂 亦称保证试剂，为一级品，纯度高，杂质极少，主要用于精密分析和科学研究，常以GR表示。
（2）分析纯试剂 亦称分析试剂，为二级品，纯度略低于优级纯，杂质含量略高于优级纯，适用于重要分析和一般性研究工作，常以AR表示。
（3）化学纯试剂 为三级品，纯度较分析纯差，但高于实验试剂，适用于工厂、学校一般性的分析工作，常以CP表示。
（4）实验试剂 为四级品，纯度比化学纯差，但比工业品纯度高，主要用于一般化学实验，不能用于分析工作，常以 LR表示。
以上按试剂纯度的分类法已在我国通用。根据化学工业部颁布的“化学试剂包装及标志”的规定，化学试剂的不同等级分别用各种不同的颜色来标志，见表1。
表1 我国化学试剂的等级及标志
2.NaNO3FeCl3AgNO3
分别有什么现象？谢谢回答！
C.MgCl2溶液和氨水D.盐酸和NaAlO2溶液
为什么
