Goof-Proof™ qPCR Master Mix is an EvaGreen dye-based hot start master mix for use in DNA melt curve analysis and in Real Time PCR (RT-PCR) applications. Goof-Proof™ is a mistake-prevention master mix which contains a low concentration of blue dye. Additionally, Goof-Proof™ includes a DNA template buffer which has a higher concentration of blue dye. With the addition of the 2X Goof-Proof™ Master Mix to the reaction, it will appear light blue (see Figure 1). And when you add the template, containing DNA template buffer, the color will turn darker blue (see Figure 2).
Goof-Proof™ allows you to see quickly and easily whether you forgot to add the master mix or the template to any of your reactions, preventing pipetting mistakes and wasted time, reagents and money. The 2X Goof-Proof™ Master Mix can also be used without the Template Buffer, if desired.
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| Figure1: 1x Goof-Proof™Master Mix | Figure 2: 1x Goof-Proof™ Master Mix after adding 1X Goof-Proof™ Template Buffer |
Kit components
- Goof-Proof™ 2X Master Mix -
- Goof-Proof™ Template Buffer -
The 2X Master Mix contains GoldBio HotStart Taq DNA Polymerase, dNTPs and a low concentration of an inert blue dye. The dye helps the user distinguish between empty wells and wells containing master mix.
The 40X Template Buffer contains a high concentration of inert blue dye. Adding the template buffer to the reaction mix makes it "Goof-Proof" to track which wells have had template added to the PCR reactions. Template Buffer is not required for the Master Mix to function, but is designed to help alleviate mistakes with template addition.
Note: Goof-Proof™ qPCR Master Mix does not include ROX reference dye. For instruments that require either a low or high concentration of ROX reference dye, we offer Goof-Proof™ qPCR Master Mix with Rox (Catalog # G-705) which includes a separate vial of ROX Reference dye, and instructions for how much ROX to add depending on which protocol and instrument you are using.
Storage/Handling: Store the kit at -20°C.
Product may be shipped on blue ice without reducing performance. Please place at the recommended storage conditions upon receipt of the product.
GoldBio活体成像技术：早在1999年由美国哈佛大学Weissleder博士率先提出了分子影像学(molecularimaging，MI)的概念，即应用影像学的方法对活体状态下的生物过程进行细胞和分子水平的定性和定量研究。活体成像便是基于分子影像学孕育而生的，通过这个成像系统，可以观测活体动物体内肿瘤的生长及转移，感染性疾病的发展进程，特定基因的表达等生物学过程。活体成像技术主要采用生物发光（bioluminescence）与荧光（fluorescence）两种技术。★生物发光是用荧光素酶基因标记细胞或DNA。★荧光技术则采用荧光报告基团（GFP、RFP,Cyt及dyes等）进行标记。★这一技术对肿瘤微小转移灶的检测灵敏度极高，不涉及放射性物质和方法,非常安全。操作极其简单、所得结果直观、灵敏度高。
活体成像两种检测技术介绍活体成像特点优点缺点生物发光检测bioluminescence★荧光素酶（Luciferase）对基因、细胞和活体动物进行标记；★荧光素酶催化底物（例如荧光素钾盐）反应后，会产生化学发光。这种光是由化学反应而来，不需要激发光；★标记方法是通过克隆技术，将荧光素酶的基因插入到预期观察的细胞染色体内，通过对克隆细胞进行筛选，培养出能稳定表达荧光素酶的细胞株。再将细胞株转移至特定的小鼠体内形成模型。★特异性强，无自发荧光；★高灵敏度，在体内可检测到几百个细胞，检测的深度在3-100px；★定量精确 ★信号较弱，检测时间较长；★仪器精密度要求较高；★细胞或基因需要转基因标记；★不可用于人体，不适用于抗体、多肽等标记荧光检测fluorescence★采用荧光报告基因(GFP、RFP等)或荧光染料进行标记；★需要外接激发光源，利用报告基因、荧光蛋白质或染料产生的荧光，就可以形成体内的生物光源。★荧光染料、蛋白标记能力强；★信号强，成像速度快，操作简便，实验成本较低；★未来可用于人；★适用范围广，可以是动物、细胞、微生物，也可以是抗体、药物、纳米材料等。★存在自发荧光，影响灵敏度；★光容易被动物组织吸收；★检测深度受限；★背景光干扰，定量准确度低
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请问下有无同学需要H37RA的？我是做EAE模型的，上个月购买了BDDifco公司的H37RA（货号），因为购买的时候只能整盒6支购买，但我们用不了那么多，所以想问问有无同学需要的，100mg/支，800元/支或用等价试剂交换。地址广州。有需要的请私信，谢谢！
A、Na2CO3HClH2SO4NaNO3
B、K2CO3H2SO4HNO3BaCl2
C、HClHNO3AgNO3NaCl
D、NaOHFeCl3MgCl2BaCl2
我想检测血管组织中的钙离子浓度，不知道哪个公司有试剂盒
2.NaHCO3,Ba(OH)3,H2SO4
3.HCL,NaAlO2,NaHSO4
4.Ca(OH)2,Na2CO3,BaCO3
谢谢了
要原因
bhclna2so4nano3na2co3
chclnaohna2co3nacl
dba(oh)2nahco3alcl3nahso4
C.MgCl2溶液和氨水D.盐酸和NaAlO2溶液
为什么
