HistonePeptideH323:42R26me2a_K27ac(H3.3C)
ProductCode:SP-His_0704
C-terminallybiotinylatedsyntheticHistonepeptidecarrying2modifications(R26me2a,K27ac)derivedfromhumanHistoneH3,H3.3C(Swiss-ProtId(s):Q6NXT2).
Aminoacidsequence:Ac-KAA-Arg(Me2a)-Lys(Ac)-STPSTCGVKPHRYRP-Lys(Biotinoyl)-NH2
Amount:1vialcontaining10nmol(appr.35µg)
Purity:>70%(HPLC-MS)
Deliveryformat:Freeze-driedinambertypeglassvials
Application(s):WesternBlot,Bindingexperiments,ELISA,Enzymekinetics,Pulldownassays,SubstrateSpecifity
Indication(s)/Topic(s):Cancer,Phosphatase,Kinase,Methyltransferase,Acetyltransferase,Histones,Phosphatase,Generegulation&expression,Epigenetics,Cellsignaling,Nucleosome,Aging
Deliverytime:2-5days
JPT"sHistoneCodePeptides
Ashistonesareamajorfocusofepigenetics,JPT"scomputationalscientistscompiledauniquelibraryofsyntheticposttranslationallymodifiedandunmodifiedhumanhistonepeptidesthatisnowavailableasindividualbiotinylatedpeptides.Thepeptidelibraryconsistsofmorethansevenhundred20merpeptidesfrom29histonefamilies(e.g.H2A[1,19,85...],H2B[1,22,42...],H3[1,23,43...],H4[1,14,44...]).PeptidescarryPTMsatreportedsites(e.g.acetylation,methylation,phosphorylation,citrullination)invariouscombinationsrepresentingnaturalvariants.
JPT"shighlyskilledandcommittedscientificstaffensuresthatthemostappropriatemethodsandtechniquesareselectedforeverysynthesisproject.AllofJPT"scatalogpeptidesareprovidedwithHPLC-MSanalysestoconfirmtheidentityanddemonstratethehighqualityofourpeptides.
BenefitsofJPT"sHistoneCodePeptides
-WholeproductionperformedinaregulatedEuropeanenvironmentaccordingtoDINENISO9001:2015&GCLPstandards;auditswelcome!
-Forhigh-throughputscreeningthewholeHistoneCodePeptidelibraryisalsoavailabledisplayedonpeptidemicroarrays
-ProvisionoffreezedriedaliquotsforenhancedstABIlity
-Proventrackrecordformanyapplications
HistonePeptideH323:42R26me2a_K27ac(H3.3C)
SelectedReferencesforHistonePeptideTools
"HistoneH2AandH4N-TerminalTailsarePositionedbytheMEP50WD-RepeatProteinforEfficientMethylationbythePRMT5ArginineMethyltransferase"
Burgosetal.,J.Biol.Chem.(2015)-PMID:25713080
"H3K23me2isaNewHeterochromaticMarkinCaenorhaBDitiselegans"
Vandammeetal., NucleicAcidsRes.(2015)-PMID:26476455
"StructureoftheArginineMethyltransferasePRMT5-MEP50RevealsaMechanismforSubstrateSpecificity"
Hoetal.PLOSONE(2013)-PMID:23451136
"UseofHigh-DensityHistonePeptideArraysforParsingtheSpecificityofaHistone-ModifyingEnzymeComplex"
Shechteretal,ApplicationNote(2013)(fulltext)
"ComprehensivePeptideMicroarraysforHistoneResearch"
Schnatbaumetal,32thEPS(2012)(fulltext)
ApplicationNotes
"UseofHigh-DensityHistonePeptideArraysforParsingtheSpecificityofaHistone-ModifyingEnzymeComplex"
Shechteretal.ApplicationNote(2013)(fulltext)
"ComprehensiveCharacterizationofAntibodiesDirectedtowardsEpigeneticHistone-Modifications"
Maschetal.ApplicationNote(2015)(fulltext)
TestimonialforHistoneCodePeptideMicroarray
"JPT"snewextremelyhigh-densityhistonepeptidearrayisadramaticleapforwardforanalysisofthehistonecodehypothesis.Thecompletesequencecoverageacrosscorehistonesandhistonevariantsaswellasthepresenceofcommonandrarehistonepost-translationalmodifications "aloneandincombination"makesthisanunparalleledtoolforstudyingthe"writers"and"readers"ofthehistonecode.MylaboratoryhasusedthesearraystoaskthesequenceandPTMdependenceofahistonewriterenzymeandwehavediscoveredanactivityspecifyingcodethatwillkeepusbusyforyearstocome."
DavidShechter,PhD,DepartmentofBiochemistry,AlbertEinsteinCollegeofMedicine,YU(NY,USA)
HistonePeptideH323:42R26me2a_K27ac(H3.3C)
| HistoneFamily | HistoneH3 |
| Modification | 2Modifications |
| SequencePosition | Tail(1-42) |
| Isoform | H3.3C |
| Biotinylation | BiotinylatedPeptide |
| Application | ELISA |
| ProteinName | Histones |
| Organism | Homosapiens(Human) |
| Indication/Topic | Acetyltransferase Epigenetics Kinase |
| Numberofpeptides | 1 |
FurtherInformation:
| Amount | 1vialcontaining10nmol(appr.35µg) |
| Specifications | C-terminallybiotinylatedsyntheticHistonepeptidecarrying2modifications(R26me2a,K27ac) |
| Documentation | ProductDatasheet |
| Swiss-ProtID | Q6NXT2 |
| Sequence | Ac-KAA-Arg(Me2a)-Lys(Ac)-STP STCGVKPHRYRP-Lys(Biotinoyl)- NH2 |
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（1）优级纯试剂 亦称保证试剂，为一级品，纯度高，杂质极少，主要用于精密分析和科学研究，常以GR表示。
（2）分析纯试剂 亦称分析试剂，为二级品，纯度略低于优级纯，杂质含量略高于优级纯，适用于重要分析和一般性研究工作，常以AR表示。
（3）化学纯试剂 为三级品，纯度较分析纯差，但高于实验试剂，适用于工厂、学校一般性的分析工作，常以CP表示。
（4）实验试剂 为四级品，纯度比化学纯差，但比工业品纯度高，主要用于一般化学实验，不能用于分析工作，常以 LR表示。
以上按试剂纯度的分类法已在我国通用。根据化学工业部颁布的“化学试剂包装及标志”的规定，化学试剂的不同等级分别用各种不同的颜色来标志，见表1。
表1 我国化学试剂的等级及标志
bhclna2so4nano3na2co3
chclnaohna2co3nacl
dba(oh)2nahco3alcl3nahso4
刚入这行，谢谢大家
A.H2SO4Na2SO4AgNO3BaCl2.
B.NaOHNa2CO3NaHSO4MgCl2
C.CaCl2NaNO3MgSO4BaCl2
D.HNO3KOHKClK2CO3
