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Biosensis/Fluoro-Jade C (FJC) Ready-to-Dilute Staining Kit for identifying Degenerating Neurons (Trial size)/TR-100-FJT/1 Ea_蚂蚁淘,【正品极速】生物医学科研用品轻松购|ebiomall 蚂蚁淘商城
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Biosensis/Fluoro-Jade C (FJC) Ready-to-Dilute Staining Kit for identifying Degenerating Neurons (Trial size)/TR-100-FJT/1 Ea
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Biosensis/Fluoro-Jade C (FJC) Ready-to-Dilute Staining Kit for identifying Degenerating Neurons (Trial size)/TR-100-FJT/1 Ea
品牌 / 
Biosensis
货号 / 
TR-100-FJT
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DescriptionThecausesandeffectsofneuronaldegenerationareofmajorinteresttoawidevarietyofneuroscientists.Parallelingthisgrowinginterestisanincreasingnumberofmethodsapplicabletothedetectionofneuronaldegeneration.Fluoro-Jade®Cstainsalldegeneratingneuronsregardlessofspecificinsultormechanismofcelldeath.Fluoro-JadeCexhibitsthegreatestsignaltobackgroundratio,aswellasthehighestresolution.Thistranslatestoastainofmaximalcontrastandaffinityfordegeneratingneurons.Thismakesitidealforlocalisingnotonlydegeneratingnervecellbodiesbutalsodistaldendrites,axonsandterminals.ThedyeishighlyresistanttofADIngandiscompatIBLewithvirtuallyallhistologicalprocessingandstainingprotocols.
ApplicationsTheFluoro-JadeC"ReadytoDilute"(RTD™)StainingKitprovidesaneasytouseassortmentofFluoro-JadeC,DAPI,sodiumhydroxideandpotassiumpermanganateinliquidform.Followingourdetailedprotocol,Fluoro-JadeClabelleddegeneratingneuronsarevisualisedwithbluelightexcitationwhileDAPIcounterstainedcellnucleiarevisualisedwithultra-violetIllumination.TheFluoro-JadeCStainingKitcanbeusedonallkindsofpreservedtissues,includingfresh-frozen,paraformaldehydeorformalinfixed,andformalinfixed,paraffin-embeddedtissues.
CommentsMATERIALSPROVIDED

SodiumHydroxide,SolutionA(Dilute1:10priortouse)-20mL
PotassiumPermanganate,SolutionB(Dilute1:10priortouse)-20mL
Fluoro-JadeC,SolutionC(Dilute1:10priortouse)-20mL
DAPI,SolutionD(AddtodilutedFluoro-JadeC)-20mL

EQUIPMENTANDREAGENTSNEEDED
Gelatincoatedmicroscopeslides
Stainingdishes/Coplinjars
Coverslips
DPXmountingmedia
Slidewarmer
Convectionoven
Distilledwater
Ethanol
Xylene


FinalworkingconcentrationsofFJC:0.0001%
FinalworkingconcentrationofKMnO4:0.06%

NUMBEROFSLIDESPROCESSED:

Theactualnumberofslidesprocessedbythiskitwilldependlargelyuponthevesselthatisusedtoincubatetheslides.IfusingastandardCoplinJar,itscapacityis50mLandtypicallyholds5slidesperjar.Ifusingsuchadevice,then80-100slidesstainedper50mlofworkingsolution(or,5mlofstocksolution)couldbeprocessedinoneday.NotethediluteddyeisNOTstableandwillnotstoreovernight.Itisbesttousefreshlydiluteddyeeachtimeanexperimentalbatchisstarted.
SpecificityDegeneratingneurons,andneuronaldegeneration.Thereisnospecificstaininginnormalhealthybrain.
Cross-reactivityNote:SomeresearchersundersomeconditionsreportbloodvesselstainingwithFluoroJade.ThismaybebecauseFluoroJadeisananalogueofeosin(whichstainsbloodcells).Ingeneral,goodperfusionandpreparationofthetissueshouldhelppreventbloodvesselstainingbutitmaynotbepossibletoeliminateitentirely.Inourexperienceitisgenerallypossibletodistinguishneuronalfrombloodvesselsstainingbyeye.
FormThereagentsintheFluoroJadekitareallsuppliedinaliquidformatandareready-to-dilute.
AppearanceFJCvisualizationisaccomplishedusingbluelightora488nmLaser.ExcitationPeak:495nmEmissionPeak:521nmFiltersystemforvisualizing:Fluorescein/FITC
ReconstitutionDilutesolutionsasdirectedintheprotocolinstructions.Sometimessmallprecipitatesmaybepresentinthestockordilutedsolutions.Completemixingofthedilutedsolutionsusuallydissolvestheprecipitates.Theprecipitates,ifnotremoved,donotusuallycauseanydifficultiesifthewashingstepsarefollowedasinstructed.Optional:ForentirelycleansolutionsBiosensisrecommendsfilteringthedilutedsolutionthoughethanolandNaOHcompatiblesyringeorvacuumfilterdevicespriortocontactwithtissueslides.
StorageTheunopenedkitcanbestoredforupto6monthsat2-8ºCafterthedateofreceipt.Thekitandcomponentsshouldbestoredprotectedfromlight.DilutedFJCdyesolutionsarenotstableandshouldbeusedwithin4hoursofmaking.Theotherdilutedsolutionscanbereusedandstoredforupto48hoursifrefrigeratedandprotectedfromlight.Bestresultsrequirefreshlydilutedsolutions.Werecommendusingaseptictechniqueswhenhandlingthereagentstoavoidbacterialgrowthandcontamination.

TheFJCReadytodilutekitisshippedambientandstableatroomtemperatureduringtransport.Refrigerateuponarrival,donotfreeze.
ExpiryDateUnopenedkit6monthsat2-8ºCprotectedfromlight.SeeStorageinstructionsforworkingsolutionsrecommendations.
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KononenkoNL,ClaßenGA,KuijpersM,PuchkovD,MaritzenT,TempesA,MalikAR,SkaleckaA,BeraS,JaworskiJ,HauckeV(2017)"RetrogradetransportofTrkB-containingautophagosomesviatheadaptorAP-2mediatesneuronalcomplexityandpreventsneurodegeneration."NatCommun.8:14819Application:IF.Species:Mouse

LianYD,ChenZX,ZhuKR,SunSY,ZhuLP(2017)"Effectofequipotentdosesofbupivacaineandropivacaineinhigh‐fatdietfedneonatalrodentmodel."BrazJAnesthesiol.67(2):131-8Application:IF.Species:Rat

MamoJCL,LamV,GilesC,CoulsonSH,FimognariN,MooranianA,Al-SalamiH,TakechiR(2017)"Antihypertensiveagentsdonotpreventblood–brainbarrierdysfunctionandcognitivedeficitsindietary-inducedobesemice."IntJObes(Lond).[Epubaheadofprint]Application:IF.Species:Mouse

IwasaK,YamamotoS,YagishitaS,MaruyamaK,YoshikawaK(2017)"Excitotoxicity-inducedprostaglandinD2productioninducessustainedmicroglialactivationanddelayedneuronaldeath."JLipidRes.58(4):649-55Application:IF.Species:Rat

XieZ,HuangL,EnkhjargalB,ReisC,WanW,TangJ,ChengY,ZhangJH(2017)"IntranasaladmiNISTrationofrecombinantNetrin-1attenuatesneuronalapoptosisbyactivatingDCC/APPL-1/AKTsignalingpathwayaftersubarachnoidhemorrhageinrats."Neuropharmacology.http://dx.doi.org/10.1016/j.neuropharm.2017.03.025[Inpress]Application:IF.Species:Rat

KatayamaY,InabaT,NitoC,SudaS,UedaM(2016)"Neuroprotectiveeffectsofclarithromycinagainstneuronaldamageincerebralischemiaandinculturedneuronalcellsafteroxygen-glucosedeprivation."LifeSci.PMID:27825902[Epubaheadofprint]Application:IF.Species:Rat

YangLY,GreigNH,HuangYN,HsiehTH,TweedieD,YuQS,HofferBJ,LuoY,KaoYC,WangJY(2016)"Post-traumaticadministrationofthep53inactivatorpifithrin-αoxygenanaloguereduceshippocampalneuronallossandimprovescognitivedeficitsafterexperimentaltraumaticbraininjury."NeurobiolDis.PMID:27553877[Epubaheadofprint]Application:IF.Species:Rat

IzumidaH,TakagiH,FujisawaH,IwataN,NakashimaK,TakeuchiS,IwamaS,NambaT,KomatuY,KaibuchiK,OisoY,ArimaH,SugimurY(2016)"NMDAreceptorantagonistpreventscelldeathinthehippocampaldentategyrusinducedbyhyponatremiaaccompanyingadrenalinsufficiencyinrats."ExpNeurol.PMID:27527984[Epubaheadofprint]Application:IF.Species:Rat

DixonBJ,ChenD,ZhangY,FloresJ,MalaguitJ,NowrangiD,ZhangJH,TangJ.(2016)"IntranasalAdministrationofInterferonBetaAttenuatesNeuronalApoptosisviatheJAK1/STAT3/BCL-2PathwayinaRatModelofNeonatalHypoxic-IschemicEncephalopathy."ASNNeuro.PMID:276838778(5)Application:IF.Species:Rat

WangJY,HuangYN,ChiuCC,TweedieD,LuoW,PickCG,ChouSY,LuoY,HofferBJ,GreigNH,WangJY(2016)"Pomalidomidemitigatesneuronalloss,neuroinflammation,andbehavioralimpairmentsinducedbytraumaticbraininjuryinrat."JNeuroinflammation.PMID:2735305313(1):168Application:IF.Species:Rat

WangG,ManaenkoA,ShaoA,OuY,YangP,BudbazarE,NowrangiD,ZhangJH,TangJ(2016)"Low-densitylipoproteinreceptor-relatedprotein-1facilitateshemescavengingafterintracerebralhemorrhageinmice."JCerebBloodFlowMetab.PMID:27317656[Epubaheadofpring]Application:IF.Species:Mouse

SafdariaBK,SiabTC,WattchowDA,SmidSD(2016)"Effectsofpro-inflammatorycytokines,lipopolysaccharideandCOX-2mediatorsonhumancolonicneuromuscularfunctionandepithelialpermeability."Cytokine.PMID:2717709283:231-8Application:IF.Species:Human

LianYD,ChenZX,ZhuKR,SunSY,ZhuLP(2016)"Effectofequipotentdosesofbupivacaineandropivacaineinhigh-fatdietfedneonatalrodentmodel."BrazilianJournalofAnesthesiology[InPress]Application:IF.Species:Rat

LaunayPS,ReboussinE,LiangH,KessalK,GodefroyD,RosteneW,SahelJA,BaudouinC,ParsadaniantzSM,LeGoazigoAR(2016)"Ocularinflammationinducestrigeminalpain,peripheralandcentralneuroinflammatorymechanisms."NeuroBIOLOGyofDisease.PMID:2674721188:16-28Application:IH.Species:Mouse

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HayashidaK,SanoM,KamimuraN,YokotaT,SuzukiM,OhtaS,FukudaK,HoriS(2014)"Hydrogeninhalationduringnormoxicresuscitationimprovesneurologicaloutcomeinaratmodelofcardiacarrestindependentlyoftargetedtemperaturemanagement."Circulation.PMID:25366995130(24):2173-80Application:IH.Species:Rat
ReagentKitprotocolClick to download the protocolTR-100-FJT_January2017_protocol.pdf
MSDSClick to download the Material Safety Data SheetMSDS_TR-100-FJ_as_at_May2017.pdf
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FeCI3、NaOH、NaCI、HNO3。
只用胶头滴管和试管,不用其他试剂就可以区别的下列溶液(浓度均为)是(   )A.和B.稀和C.和D.和盐酸
1.NaHCO3NaHSO4Ba(NO3)2

2.NaNO3FeCl3AgNO3
分别有什么现象?谢谢回答!
NaOH溶液、CuSO4溶液、Na2SO4溶液、MgSO4溶液
碧云天试剂盒有用过的吗

想请问一下用DNA试剂盒离心柱法提取的DNA浓度一般是多少啊?

我用DNA试剂盒离心柱法提取的DNA浓度只有一二十是不是太低了做PCR酶切之类的能做出来么?

谢谢!

例:1。硝酸钡2。硝酸银3。氯化钠4。氯化铜四种溶液检验出的顺序。

通过解答,教会我,谢谢
乙醇,乙醛,盐酸,CU(OH)2悬浊液
下列各组溶液里,不用任何其他试剂就能鉴别的是
A.H2SO4Na2SO4AgNO3BaCl2.
B.NaOHNa2CO3NaHSO4MgCl2
C.CaCl2NaNO3MgSO4BaCl2
D.HNO3KOHKClK2CO3
最近发现一个问题一般生化仪至少能检测二三十个项目!那么问题是这么多项目要加的试剂R1,R2,算下来也得六七十个瓶瓶罐罐,每天加试剂,倒过来倒过去的,很容易把试剂加错吧?所以就想问一下各位平时工作中有......