描述
TheThermoScientificPierceBCAProteinAssayKitisatwo-component,high-precision,detergent-compatIBLeassayreagentsettomeasure(A562nm)totalproteinconcentrationcomparedtoaproteinstandard.
FeaturesoftheBCAProteinAssayKit:
• Colorimetric—estimatevisuallyormeasurewithastandardspectrophotometerorplatereader(562nm)
• Excellentuniformity—exhibitslessprotein-to-proteinvariationthandye-bindingmethods
• Compatible—unaffectedbytypicalconcentrationsofmostionicandnonionicdetergents
• Moderatelyfast—mucheasierandfourtimesfasterthantheclassicalLowrymethod
• Highlinearity—linearworkingrangeforBSAequals20to2000µg/mL
• Sensitive—detectdownto5µg/mLwiththeenhancedprotocol
Usedinmorelabsthananyotherdetergent-compatibleproteinassay,PierceBCAReagentsprovideaccuratedeterminationofproteinconcentrationwithmostsampletypesencounteredinproteinresearch.ThePierceBCAAssaycanbeusedtoassessyieldsinwholecelllysatesandaffinity-columnfractions,aswellastomonitorproteincontaminationinindustrialapplications.Comparedtomostdye-bindingmethods,theBCAAssayisaffectedmuchlessbyproteincompositionaldifferences,providinggreaterprotein-to-proteinuniformity.
Applications:
•Studyingprotein:proteininteractions
•Measuringcolumnfractionsafteraffinitychromatography
•Estimatingpercentrecoveryofmembraneproteinsfromcellextracts
•High-throughputscreeningoffusionproteins
HowtheBCAProteinAssayDetectsProtein:
TheBCAProteinAssaycombinesthewell-knownreductionofCu2+ toCu1+ byproteininanalkalinemediumwiththehighlysensitiveandselectivecolorimetricdetectionofthecuprouscation(Cu1+)bybicinchoninicacid.Thefirststepisthechelationofcopperwithproteininanalkalineenvironmenttoformalightbluecomplex.Inthisreaction,knownasthebiuretreaction,peptidescontainingthreeormoreaminoacidresiduesformacoloredchelatecomplexwithcupricionsinanalkalineenvironmentcontainingsodiumpotassiumtartrate.
Inthesecondstepofthecolordevelopmentreaction,bicinchoninicacid(BCA)reactswiththereduced(cuprous)cationthatwasformedinstepone.Theintensepurple-coloredreactionproductresultsfromthechelationoftwomoleculesofBCAwithonecuprousion.TheBCA/coppercomplexiswater-solubleandexhibitsastronglinearabsorbanceat562nmwithincreasingproteinconcentrations.TheBCAreagentisapproximately100timesmoresensitive(lowerlimitofdetection)thanthepalebluecolorofthefirstreaction.
ThereactionthatleadstoBCAcolorformationisstronglyinfluencedbyfouraminoacidresidues(cysteineorcystine,tyrosine,andtryptophan)intheaminoacidsequenceoftheprotein.However,unliketheCoomassiedye-bindingmethods,theuniversalpeptidebackbonealsocontributestocolorformation,helpingtominimizevariABIlitycausedbyproteincompositionaldifferences.
Formoreinformation,seethearticle"ChemistryofProteinAssays"intheProteinMethodsLibrary.
RelatedProducts
Pierce™BCAProteinAssayReagentA
Pierce™BCAProteinAssayReagentB
Pierce™BCASolid
AmpuleBreakers
规格
| AssayType: | ProteinQuantitationAssay |
|---|---|
| DetectionMethod: | Colorimetric |
| ForUseWith(Equipment): | MicroplateReader, Spectrophotometer |
| ProductLine: | Pierce™ |
| ProductSize: | 1L |
| Technique: | Absorbance, Solution-basedDetection |
内容及储存
SufficientFor:500tubeassaysor5000microplateassays
•BCAReagentA,2x500mL
•BCAReagentB,25mL
•AlbuminStandardAmpules,2mg/mL,10x1mL
Storeatroomtemperature
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
2.NaHCO3,Ba(OH)3,H2SO4
3.HCL,NaAlO2,NaHSO4
4.Ca(OH)2,Na2CO3,BaCO3
谢谢了
要原因
刚入这行，谢谢大家
请问下有无同学需要H37RA的？我是做EAE模型的，上个月购买了BDDifco公司的H37RA（货号），因为购买的时候只能整盒6支购买，但我们用不了那么多，所以想问问有无同学需要的，100mg/支，800元/支或用等价试剂交换。地址广州。有需要的请私信，谢谢！
暂无品牌分类
