Accuris qMAX cDNA Synthesis Kit
cDNA is a vital research tool for Molecular Biologists. The qMax DNA Synthesis Kit from Accuris provides a quick, accurate and reproducible way to obtain high quality cDNA with a complete sequence representation for 4pg to 2µg of total RNA or mRNA.
· Rapidly generate high quality qPCR ready cDNA
· Unbiased, complete sequence representation
· Thermostable qMAX RT with RNase inhibitor
· Optimized for use across a wide dynamic range
· Two tube format for easy reaction setup.
The centerpiece of the qMax cDNA Synthesis Kit is qMax RT - a thermostable reverse transcriptase that allows reactions to be carried out at higher temperatures, thus eliminating the problems created by secondary structure in the RNA. A potent RNase inhibitor maintains integrity of the starting material. Unbiased representation and synthesis are ensured by a precise mix of anchored oligo (dT) and hexamer primers in the 5X buffer system. Buffer and enzyme are supplied in separate tubes to allow for negative control reactions.
Accuris qMax qPCR mixes are the ideal partner for quantitative PCR of cDNA.
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请问一般的实验室可以进行ELIspot吗?(我可以买到现成的试剂盒,资金这个应该没有问题。)ELIspot的难点在哪里?多谢大虾们的指点!这个对我很重要,多谢!!!
用Mabteck公司的ALP标记抗体吧,配合Millipore公司的Multiscreen-IP96孔板,所有的试剂都可以减半,照样可以出非常好的结果,4*96孔的试剂盒我一直做8*96孔,但BCIP/NBT显色液没试过减半,因为它很便宜。记得当年第一次做实验就出了很好的结果,经过不断摸索,做出的蓝紫色斑点确实非常漂亮,几乎没有非特异斑点,嗯,ELISPOT是我最拿手的实验之一。
关于PBMC:
拿到血液标本后,首先我要做的是尽快分离PBMC(一般2h内),国产的分离液里天津灏洋的比较好,然后用冻存液冻存PBMC。PBMC的冻存过程比一般的细胞株要更为严格,最好用冻存器。ELISPOT试验的第一天,在包被抗体的同时,将细胞复苏,完全培养液培养过夜,第二天收集细胞并计数即可。
我想用酶联免疫斑点(ELISPOT)技术测定NK细胞活性,但不知道哪里能买到试剂盒,特向大家求助,谢谢。