| Contents | amfiEco Taq DNA Polymerase, 1u/ulamfiEco 2X Reaction Buffer |
|---|---|
| Application | Routine Robust PCR |
amfiEcoTaq DNA Polymeraseis a thermostable DNApolymerase that possesses a 5' -> 3' polymerase activityand a double-strand specific 5' ->3' exonuclease activity.2X amfiEco PCR Master buffer including dNTPs andMg++ minimises reagents handling steps and reducethe risk of contamination.
To perform PCR, simply add water, primers and templateto resuspend the reaction mixture, and cycle.For addedconvenience,amfiEcoTaq DNA Polymerasecontainsred and yellow loading dyes to allow loading of PCRproduct directly on a gel after thermal cycling, minimizingpipetting steps and providing easy visualization of sample.The red dye runs in a range between 500bp (2% gel) and1500bp(0.8% gel) and the yellow dye runs at less than10bp.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
我在做5RACE,选用TaKaRa公司的RNA反转录酶(RNaseH-),不知道这个酶好不好,有什么需要注意的。
没有序列识别的功能,他就是简单的催化相邻DNA链的5-P末端和3'-OH末端以磷酸二酯键结合的反应,需ATP作辅酶。 不仅可以催化粘性末端之间或平滑末端之间的DNA的连接,也催化DNA与RNA之间以及少数RNA之间的连接。
反转录酶(Reversetranscripatase)是以RNA为模板指导三磷酸脱氧核苷酸合成互补DNA(cDNA)的酶。哺乳类C型病毒的反转录酶和鼠类B型病毒的反转录酶都是一条多肽链。鸟类RNA病毒的反转录酶则由两上亚基结构。真核生物中也都分离出具有不同结构的反转录酶。

