Overview:
CDK9/Cyclin T2 is a member of the cyclin-dependent protein kinase (CDK) family which are known to be important regulators of cell cycle progression. Cyclin T2 and its kinase partner CDK9 are found to be subunits of the transcription elongation factor p-TEFb. The p-TEFb complex containing this cyclin has been reported to interact with, and act as a negative regulator of human immunodeficiency virus type 1 (HIV-1) Tat protein (1). The transcription-dependent interaction of P-TEFb with 7SK may therefore contribute to an important feedback loop modulating the activity of RNA Pol II (2).
Gene Aliases:
CDK9:CDC2L4, C-2k, TAK, PITALRECyclinT2:CCNT2; CYCT2
Genbank Number:
NM_001261NM_001241
References:
1. Peng, J.et.al: Identification of multiple cyclin subunits of human P-TEFb. Genes Dev. 12: 755-762, 1998.2. Nguyen, V. T.et.al: 7SK small nuclear RNA binds to and inhibits the activity of CDK9/cyclin T complexes.Nature 414: 322-325, 2001.
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我在做5RACE,选用TaKaRa公司的RNA反转录酶(RNaseH-),不知道这个酶好不好,有什么需要注意的。
没有序列识别的功能,他就是简单的催化相邻DNA链的5-P末端和3'-OH末端以磷酸二酯键结合的反应,需ATP作辅酶。 不仅可以催化粘性末端之间或平滑末端之间的DNA的连接,也催化DNA与RNA之间以及少数RNA之间的连接。
反转录酶(Reversetranscripatase)是以RNA为模板指导三磷酸脱氧核苷酸合成互补DNA(cDNA)的酶。哺乳类C型病毒的反转录酶和鼠类B型病毒的反转录酶都是一条多肽链。鸟类RNA病毒的反转录酶则由两上亚基结构。真核生物中也都分离出具有不同结构的反转录酶。

