Overview:
TAOK3 or TAO kinase 3 is a member of the serine/threonine-protein kinase family that inhibits the basal activity of Jun kinase and is negatively regulated by epidermal growth factor (EGF) (1). Overexpression ofTAOK3 may activate the ERK1/ERK2 and JNK/SAPK targets. TAOK3 is highly expressed in peripheral blood leukocytes (PBLs), thymus, spleen, kidney, skeletal muscle, heart and liver (2).
Gene Aliases:
DPK, JIK, MAP3K18, FLJ31808, DKFZp666H245
Genbank Number:
NM_016281
References:
1. Tassi E., et al: Human JIK, a novel member of the STE20 kinase family that inhibits JNK and is negatively regulated by epidermal growth factor. J. Biol. Chem. 274:33287-33295(1999).2. Yustein J.T.et.al: Comparative studies of a new subfamily of human Ste20-like kinases: homodimerization, subcellular localization, and selective activation of MKK3 and p38. Oncogene 22:6129-6141(2003).
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我在做5RACE,选用TaKaRa公司的RNA反转录酶(RNaseH-),不知道这个酶好不好,有什么需要注意的。
没有序列识别的功能,他就是简单的催化相邻DNA链的5-P末端和3'-OH末端以磷酸二酯键结合的反应,需ATP作辅酶。 不仅可以催化粘性末端之间或平滑末端之间的DNA的连接,也催化DNA与RNA之间以及少数RNA之间的连接。
反转录酶(Reversetranscripatase)是以RNA为模板指导三磷酸脱氧核苷酸合成互补DNA(cDNA)的酶。哺乳类C型病毒的反转录酶和鼠类B型病毒的反转录酶都是一条多肽链。鸟类RNA病毒的反转录酶则由两上亚基结构。真核生物中也都分离出具有不同结构的反转录酶。

