Overview:
CSK is a cytoplasmic tyrosine kinase that has been shown to downregulate the tyrosine kinase activity of the c-src through tyrosine phosphorylation of the c-src carboxy terminus (1). A yeast 2-hybrid system has been used to identify proteins associated with CSK. The Src homology-3 (SH3) domain of CSK associates with a proline-rich region of PEP, a protein-tyrosine phosphatase expressed in hemopoietic cells (2). This association is highly specific and it is speculated that PEP may be an effector and/or regulator of CSK in T cells and other hemopoietic cells.
Gene Aliases:
MGC117393
Genbank Number:
NM_004383
References:
1. Partanen, J. et al: Cyl encodes a putative cytoplasmic tyrosine kinase lacking the conserved tyrosine autophosphorylation site (Y416-src). Oncogene 6: 2013-2018, 1991.2. Cloutier, J.-F. et al: Association of inhibitory tyrosine protein kinase p50(csk) with protein tyrosine phosphatase PEP in T cells and other hemopoietic cells. EMBO J. 15: 4909-4918, 1996.
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我在做5RACE,选用TaKaRa公司的RNA反转录酶(RNaseH-),不知道这个酶好不好,有什么需要注意的。
没有序列识别的功能,他就是简单的催化相邻DNA链的5-P末端和3'-OH末端以磷酸二酯键结合的反应,需ATP作辅酶。 不仅可以催化粘性末端之间或平滑末端之间的DNA的连接,也催化DNA与RNA之间以及少数RNA之间的连接。
反转录酶(Reversetranscripatase)是以RNA为模板指导三磷酸脱氧核苷酸合成互补DNA(cDNA)的酶。哺乳类C型病毒的反转录酶和鼠类B型病毒的反转录酶都是一条多肽链。鸟类RNA病毒的反转录酶则由两上亚基结构。真核生物中也都分离出具有不同结构的反转录酶。

