Overview:
EPHA7 is a member of the ephrin receptor subfamily of protein-tyrosine kinases family that have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2-fibronectin types III repeats. EPHA7 mediated signaling act as a physiologic trigger for apoptosis that can alter brain size and shape by regulating the number of neural progenitors (1). The high expression of EPHA7 protein plays an important role in the malignancy transformation, invasion progression and metastasis of primary hepatocellular carcinoma (2).
Gene Aliases:
CEK11, EHK3, HEK11, MDK1
Genbank Number:
BC026153
References:
1. Depaepe, V. et.al: Ephrin signalling controls brain size by regulating apoptosis of neural progenitors. Nature 435: 1244-1250, 2005.2. Zhang SJ. et.al: Expression of EphA7 protein in primary hepatocellular carcinoma and its clinical significance. Zhonghua Wai Ke Za Zhi. 2010 Jan 1;48(1):53-6.
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我在做5RACE,选用TaKaRa公司的RNA反转录酶(RNaseH-),不知道这个酶好不好,有什么需要注意的。
没有序列识别的功能,他就是简单的催化相邻DNA链的5-P末端和3'-OH末端以磷酸二酯键结合的反应,需ATP作辅酶。 不仅可以催化粘性末端之间或平滑末端之间的DNA的连接,也催化DNA与RNA之间以及少数RNA之间的连接。
反转录酶(Reversetranscripatase)是以RNA为模板指导三磷酸脱氧核苷酸合成互补DNA(cDNA)的酶。哺乳类C型病毒的反转录酶和鼠类B型病毒的反转录酶都是一条多肽链。鸟类RNA病毒的反转录酶则由两上亚基结构。真核生物中也都分离出具有不同结构的反转录酶。

