Overview:
EPHB2 is a member of the Eph family of receptor tyrosine kinases that mediate neurodevelopmental processes such as boundary formation, axon guidance, vasculogenesis, and cell migration. EPHB2 has been shown to associate with the non-receptor tyrosine kinase Abl and activated EPHB2 causes tyrosine phosphorylation of Abl and regulates its activity (1). EPHB2 is overexpressed in a number of tumors particularly glioblastoma and this increases glioma cell migration and invasion (2).
Gene Aliases:
DRT, ERK, Hek5, EPHT3, Tyro5, CAPB, PCBC
Genbank Number:
NM_004442
References:
1. Yu, H.H. et al: Multiple signaling interactions of Abl and Arg kinases with the EphB2 receptor. Oncogene. 2001 Jul 5;20(30):3995-4006.2. Nakada, M. et al: The phosphorylation of EphB2 receptor regulates migration and invasion of human glioma cells. Cancer Res. 2004 May 1;64(9):3179-85.
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我在做5RACE,选用TaKaRa公司的RNA反转录酶(RNaseH-),不知道这个酶好不好,有什么需要注意的。
没有序列识别的功能,他就是简单的催化相邻DNA链的5-P末端和3'-OH末端以磷酸二酯键结合的反应,需ATP作辅酶。 不仅可以催化粘性末端之间或平滑末端之间的DNA的连接,也催化DNA与RNA之间以及少数RNA之间的连接。
反转录酶(Reversetranscripatase)是以RNA为模板指导三磷酸脱氧核苷酸合成互补DNA(cDNA)的酶。哺乳类C型病毒的反转录酶和鼠类B型病毒的反转录酶都是一条多肽链。鸟类RNA病毒的反转录酶则由两上亚基结构。真核生物中也都分离出具有不同结构的反转录酶。

