Overview:
EPHB3 is a member of the Ephrin receptor family and is expressed during embryonic development in multiple regions of the central nervous system. In adult brain, EPHB3 is expressed in the cerebellum, raphe pallidus, hippocampus, entorhinal cortex, and both motor and sensory cortices (1). EPHB3 is involved in the maintenance of mature neuronal connections and/or re-arrangement of synaptic connections during late stages of development. EPHB3 plays a role in the regulation of cell adhesion and migration, and the catalytic activity of EPHB3 is required for inhibition of integrin-mediated cell adhesion (2).
Gene Aliases:
ETK2; HEK2; Cek10; Mdk5; TYRO6
Genbank Number:
NM_004443
References:
1.Willson, CA. et al: EphB3 receptor and ligand expression in the adult rat brain. J Mol Histol. 2006 Nov;37(8-9):369-80.2.Miao, H. et al: Inhibition of integrin-mediated cell adhesion but not directional cell migration requires catalytic activity of EphB3 receptor tyrosine kinase. Role of Rho family small GTPases. J Biol Chem. 2005 Jan 14;280(2):923-32.
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我在做5RACE,选用TaKaRa公司的RNA反转录酶(RNaseH-),不知道这个酶好不好,有什么需要注意的。
没有序列识别的功能,他就是简单的催化相邻DNA链的5-P末端和3'-OH末端以磷酸二酯键结合的反应,需ATP作辅酶。 不仅可以催化粘性末端之间或平滑末端之间的DNA的连接,也催化DNA与RNA之间以及少数RNA之间的连接。
反转录酶(Reversetranscripatase)是以RNA为模板指导三磷酸脱氧核苷酸合成互补DNA(cDNA)的酶。哺乳类C型病毒的反转录酶和鼠类B型病毒的反转录酶都是一条多肽链。鸟类RNA病毒的反转录酶则由两上亚基结构。真核生物中也都分离出具有不同结构的反转录酶。

