Overview:
FMS is a proto-oncogene that encodes the tyrosine kinase transmembrane receptor for colony stimulating factor 1 (CSF1). FMS is homodimeric that contains a so-called kinase insert domain and is a member of the CSF1/PDGF receptor family of tyrosine-protein kinases. FMS mediates most if not all of the biological effects of CSF1 which control the production, differentiation, and function of cell of the monocyte/macrophage lineage (1). Mutations in FMS have been associated with providing sustained signals for cell growth and a predisposition to myeloid malignancy (2).
Gene Aliases:
CSF1R, CSFR, FIM2, C-FMS, CD115
Genbank Number:
NM_005211
References:
1. Sherr, C J.: Regulation of mononuclear phagocyte proliferation by colony-stimulating factor-1. Int J Cell Cloning. 1990 Jan;8 Suppl 1:46-602. Follows, G A. et al: c-FMS chromatin structure and expression in normal and leukaemic myelopoiesis. Oncogene. 2005 May 19;24(22):3643-51.
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我在做5RACE,选用TaKaRa公司的RNA反转录酶(RNaseH-),不知道这个酶好不好,有什么需要注意的。
没有序列识别的功能,他就是简单的催化相邻DNA链的5-P末端和3'-OH末端以磷酸二酯键结合的反应,需ATP作辅酶。 不仅可以催化粘性末端之间或平滑末端之间的DNA的连接,也催化DNA与RNA之间以及少数RNA之间的连接。
反转录酶(Reversetranscripatase)是以RNA为模板指导三磷酸脱氧核苷酸合成互补DNA(cDNA)的酶。哺乳类C型病毒的反转录酶和鼠类B型病毒的反转录酶都是一条多肽链。鸟类RNA病毒的反转录酶则由两上亚基结构。真核生物中也都分离出具有不同结构的反转录酶。

