Overview:
FRK (fyn-related kinase) or Rak is a nuclear tyrosine kinaseand member of the Src sub-family. Restricted expression of FRK is detected in a broad range of cell lines with highest levels in epithelial cells. Increased expression of FRK has been shown in breast and renal cell carcinoma cell lines. In addition the retinoblastoma tumor susceptibility gene product pRb associates with FRK in vitro and in vivo (1). Overexpression of FRK in beta-cells from the pancreas increases the susceptibility of these cells to beta-cell-toxic events (hallmark of Type I diabetes)(2).
Gene Aliases:
GTK; RAK; PTK5
Genbank Number:
NM_002031
References:
1.Craven, R J. et al: The nuclear tyrosine kinase Rak associates with the retinoblastoma protein pRb. Cancer Res. 1995 Sep 15;55(18):3969-72.2.Welsh, M. et al: The tyrosine kinase FRK/RAK participates in cytokine-induced islet cell cytotoxicity. Biochem J. 2004 Aug 15;382(Pt 1):261-8.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
我在做5RACE,选用TaKaRa公司的RNA反转录酶(RNaseH-),不知道这个酶好不好,有什么需要注意的。
没有序列识别的功能,他就是简单的催化相邻DNA链的5-P末端和3'-OH末端以磷酸二酯键结合的反应,需ATP作辅酶。 不仅可以催化粘性末端之间或平滑末端之间的DNA的连接,也催化DNA与RNA之间以及少数RNA之间的连接。
反转录酶(Reversetranscripatase)是以RNA为模板指导三磷酸脱氧核苷酸合成互补DNA(cDNA)的酶。哺乳类C型病毒的反转录酶和鼠类B型病毒的反转录酶都是一条多肽链。鸟类RNA病毒的反转录酶则由两上亚基结构。真核生物中也都分离出具有不同结构的反转录酶。

