Overview:
ROS1 is a proto-oncogene and member of the sevenless subfamily of tyrosine kinase insulin receptor genes.ROS1 is highly-expressed in a variety of tumor cell lines and functions as a growth or differentiation factor receptor.The FIG gene can fuse with the ROS1 gene in glioblastoma cell lines (1).The resulting ROS1/FIG fusion protein is a constitutively activated tyrosine kinase.Direct interaction of ROS1 and the phosphatase SHP-1 can lead to efficient downregulation of ROS1-mediated signaling (2).Binding sites in the ROS1 cytoplasmic domain display high affinity binding to the SHP-1 N-terminal SH2 domain.
Gene Aliases:
c-ros-1; MCF3; ROS
Genbank Number:
NM_002944
References:
1. Charest, A. et al: Fusion of FIG to the receptor tyrosine kinase ROS in a glioblastoma with an interstitial del (6) (q21q21). Genes Chromosomes Cancer 37: 58-71, 2003.2. Biscup, C. et al: Visualization of SHP-1-target interaction. J Cell Sci. 2004 Oct 1; 117(Pt 21):5165-78.
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我在做5RACE,选用TaKaRa公司的RNA反转录酶(RNaseH-),不知道这个酶好不好,有什么需要注意的。
没有序列识别的功能,他就是简单的催化相邻DNA链的5-P末端和3'-OH末端以磷酸二酯键结合的反应,需ATP作辅酶。 不仅可以催化粘性末端之间或平滑末端之间的DNA的连接,也催化DNA与RNA之间以及少数RNA之间的连接。
反转录酶(Reversetranscripatase)是以RNA为模板指导三磷酸脱氧核苷酸合成互补DNA(cDNA)的酶。哺乳类C型病毒的反转录酶和鼠类B型病毒的反转录酶都是一条多肽链。鸟类RNA病毒的反转录酶则由两上亚基结构。真核生物中也都分离出具有不同结构的反转录酶。

