Overview:
LRRK1, also called leucine-rich repeat kinase 1, is highly expressed in testis, placenta, prostate, and muscle, with moderate expression in brain, pancreas, small intestine, stomach, and spleen. Mutations in LRRK1 corresponding to Parkinson disease-8 -associated mutations found in LRRK2, significantly reduced LRRK1 kinase activity (1). LRRK1 expression in all mouse brain regions examined, with highest expression in olfactory bulb (2). LRRK1 functions as a GDP/GTP-binding protein and its kinase activity is stimulated upon binding of GTP to the ROC domain.
Gene Aliases:
RIPK6; Roco1; FLJ23119; KIAA1790
Genbank Number:
NM_024652
References:
1. Korr, D.et.al: LRRK1 protein kinase activity is stimulated upon binding of GTP to its Roc domain. Cell. Signal. 18: 910-920, 2006.2. Taylor, J. P.et.al: Leucine-rich repeat kinase 1: a paralog of LRRK2 and a candidate gene for Parkinson's disease. Neurogenetics 8: 95-102, 2007
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我在做5RACE,选用TaKaRa公司的RNA反转录酶(RNaseH-),不知道这个酶好不好,有什么需要注意的。
没有序列识别的功能,他就是简单的催化相邻DNA链的5-P末端和3'-OH末端以磷酸二酯键结合的反应,需ATP作辅酶。 不仅可以催化粘性末端之间或平滑末端之间的DNA的连接,也催化DNA与RNA之间以及少数RNA之间的连接。
反转录酶(Reversetranscripatase)是以RNA为模板指导三磷酸脱氧核苷酸合成互补DNA(cDNA)的酶。哺乳类C型病毒的反转录酶和鼠类B型病毒的反转录酶都是一条多肽链。鸟类RNA病毒的反转录酶则由两上亚基结构。真核生物中也都分离出具有不同结构的反转录酶。

