Overview:
HDAC3 or Histone deacetylase 3 belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex that represses transcription when tethered to a promoter. HDAC3 may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. HDAC3 can also down-regulate p53 function and thus modulate cell growth and apoptosis. Catalytic domain of HDAC4 can interact with HDAC3 via the transcriptional corepressor NCOR2 (1). RELA is a nonhistone substrate of HDAC3 and IKBA-dependent nuclear export of the HDAC3-deacetylated RELA replenishes the depleted cytoplasmic pool of latent NFKB-IKBA complexes (2).
Gene Aliases:
HD3; RPD3; RPD3-2
Genbank Number:
NM_003883
References:
1. Chen, L. et. al: Duration of nuclear NF-kappa-B action regulated by reversible acetylation. Science 293: 1653-1657, 2001.2. Fischle, W. et al: Enzymatic activity associated with class II HDACs is dependent on a multiprotein complex containing HDAC3 and SMRT/N-CoR. Molec. Cell 9: 45-57, 2002.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
我在做5RACE,选用TaKaRa公司的RNA反转录酶(RNaseH-),不知道这个酶好不好,有什么需要注意的。
没有序列识别的功能,他就是简单的催化相邻DNA链的5-P末端和3'-OH末端以磷酸二酯键结合的反应,需ATP作辅酶。 不仅可以催化粘性末端之间或平滑末端之间的DNA的连接,也催化DNA与RNA之间以及少数RNA之间的连接。
反转录酶(Reversetranscripatase)是以RNA为模板指导三磷酸脱氧核苷酸合成互补DNA(cDNA)的酶。哺乳类C型病毒的反转录酶和鼠类B型病毒的反转录酶都是一条多肽链。鸟类RNA病毒的反转录酶则由两上亚基结构。真核生物中也都分离出具有不同结构的反转录酶。

