Overview:
E3 ubiquitin-protein ligase RNF34L, also named CARP2 (Caspases-8/10 Associated RING protein 2), acts as RING‑domain E3 ligases, ubiquitinates apical caspases and targets them for proteasome-mediated degradation. CARP gene silencing inhibits tumor cell survival and increases cancer cell sensitivity to the death ligand or chemotherapy-induced apoptosis. CARP proteins target the tumor suppressor p53 for degradation. RNF34L also acts at the level of endocytic vesicles to limit the intensity of TNF-induced NF-κB activation by the regulated elimination of the adaptor protein RIP1.
Gene Aliases:
FRING; CARP2; CARP-2; RNF189; RNF34L; RIFIFYLIN
Genbank Number:
NM_001017368
References:
1. Liao, W. et al: CARP-2 is an endosome-associated ubiquitin ligase for RIP and regulates TNF-induced NF-kappaB activation. Curr Biol. 18(9):641-649, 20082. McDonald, E.R. 3rd et al: Suppression of caspase-8- and -10-associated RING proteins results in sensitization to death ligands and inhibition of tumor cell growth. PNAS. 20;101(16):6170-6175, 2004br>3. Yang, W. et al: CARPs are E3 ligases that target apical caspases and p53. Cancer Biol. Ther. 6(11):1676-1683, 2007
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我在做5RACE,选用TaKaRa公司的RNA反转录酶(RNaseH-),不知道这个酶好不好,有什么需要注意的。
没有序列识别的功能,他就是简单的催化相邻DNA链的5-P末端和3'-OH末端以磷酸二酯键结合的反应,需ATP作辅酶。 不仅可以催化粘性末端之间或平滑末端之间的DNA的连接,也催化DNA与RNA之间以及少数RNA之间的连接。
反转录酶(Reversetranscripatase)是以RNA为模板指导三磷酸脱氧核苷酸合成互补DNA(cDNA)的酶。哺乳类C型病毒的反转录酶和鼠类B型病毒的反转录酶都是一条多肽链。鸟类RNA病毒的反转录酶则由两上亚基结构。真核生物中也都分离出具有不同结构的反转录酶。

