Overview:
PIP4K2A or phosphatidylinositol-5-phosphate 4-kinase, type II, alpha, is a member of the phosphatidylinositol-5-phosphate 4-kinase family that catalyzes the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to formphosphatidylinositol-4,5-bisphosphate (1). PIP4K2A plays as essential role in the phosphoinositide signal transduction cascades as the precursor to second messengers and is involved in the regulation of secretion, cell proliferation, differentiation, and motility (2). PIP4K2A may be one of the factors related to the regulation of the beta-globin gene expression and the different levels of Hb H in alpha-thalassemic patients.
Gene Aliases:
FLJ13267; PI5P4KA; PIP5K2A; PIP5KII-alpha; PIP5KIIA; PIPK
Genbank Number:
NM_005028
References:
1. Boronenkov, I V. et.al: The sequence of phosphatidylinositol-4-phosphate 5-kinase defines a novel family of lipid kinases. J. Biol. Chem. 270: 2881-2884, 1995.2. Rameh, L E.et.al: A new pathway for synthesis of phosphatidylinositol-4,5-bisphosphate. Nature 390: 192-196, 1997.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
我在做5RACE,选用TaKaRa公司的RNA反转录酶(RNaseH-),不知道这个酶好不好,有什么需要注意的。
没有序列识别的功能,他就是简单的催化相邻DNA链的5-P末端和3'-OH末端以磷酸二酯键结合的反应,需ATP作辅酶。 不仅可以催化粘性末端之间或平滑末端之间的DNA的连接,也催化DNA与RNA之间以及少数RNA之间的连接。
反转录酶(Reversetranscripatase)是以RNA为模板指导三磷酸脱氧核苷酸合成互补DNA(cDNA)的酶。哺乳类C型病毒的反转录酶和鼠类B型病毒的反转录酶都是一条多肽链。鸟类RNA病毒的反转录酶则由两上亚基结构。真核生物中也都分离出具有不同结构的反转录酶。

