Overview:
PIP5K1A or phosphatidylinositol-4-phosphate 5-kinase 1 alpha phosphorylates phosphatidylinositol-4-phosphate to form phosphatidylinositol 4,5-bisphosphate. PIP5K1 is required for Wnt3a-induced LRP6 phosphorylation at ser1490 in mammalian cells (1). Wnt3a stimulates the formation of phosphatidylinositol 4,5-bisphosphates through 'frizzled' and 'dishevelled', the latter of which directly interacts with and activated PIP5KI. PIP5K1A-mediated PIP(2) production is crucial for HIV-1 entry and the early steps of infection in permissive lymphocytes (2). Overexpression of wild-type PI4P5K1A increases HIV-1 Env-mediated PIP(2) production and enhances viral replication in primary lymphocytes.
Gene Aliases:
RP11-68I18.9
Genbank Number:
BC007833
References:
1. Pan, W. et al: Wnt3a-mediated formation of phosphatidylinositol 4,5-bisphosphate regulates LRP6 phosphorylation. Science 321: 1350-1353, 2008.2. Barrero-Villar M, PI4P5-kinase Ialpha is required for efficient HIV-1 entry and infection of T cells. J Immunol. 2008 Nov 15;181(10):6882-8.
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我在做5RACE,选用TaKaRa公司的RNA反转录酶(RNaseH-),不知道这个酶好不好,有什么需要注意的。
没有序列识别的功能,他就是简单的催化相邻DNA链的5-P末端和3'-OH末端以磷酸二酯键结合的反应,需ATP作辅酶。 不仅可以催化粘性末端之间或平滑末端之间的DNA的连接,也催化DNA与RNA之间以及少数RNA之间的连接。
反转录酶(Reversetranscripatase)是以RNA为模板指导三磷酸脱氧核苷酸合成互补DNA(cDNA)的酶。哺乳类C型病毒的反转录酶和鼠类B型病毒的反转录酶都是一条多肽链。鸟类RNA病毒的反转录酶则由两上亚基结构。真核生物中也都分离出具有不同结构的反转录酶。

