Overview:
Thrombin is an endolytic serine protease that cleaves fibrinogen to produce insoluble active fibrin strands and fibrinopeptides A and B. This enzymatic process is essential for blood clot formation. Thrombin recognizes the consensus sequence A-B-Pro-Arg-▼-X-Y, with the first two amino acids representing hydrophobic residues and the last two amino acids representing non-acidic residues. The common recognized sequences are Leu-Val-Pro-Arg-▼-Gly-Ser and Gly-Arg-▼-Gly. Thrombin has high proteolytic specificity and hydrolytic efficiency, making it a widely used tool for cleaving purification tag from recombinant fusion proteins.
Gene Aliases:
F2; PT; RPRGL2; THPH; coagulation Factor II; Factor IIa
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我在做5RACE,选用TaKaRa公司的RNA反转录酶(RNaseH-),不知道这个酶好不好,有什么需要注意的。
没有序列识别的功能,他就是简单的催化相邻DNA链的5-P末端和3'-OH末端以磷酸二酯键结合的反应,需ATP作辅酶。 不仅可以催化粘性末端之间或平滑末端之间的DNA的连接,也催化DNA与RNA之间以及少数RNA之间的连接。
反转录酶(Reversetranscripatase)是以RNA为模板指导三磷酸脱氧核苷酸合成互补DNA(cDNA)的酶。哺乳类C型病毒的反转录酶和鼠类B型病毒的反转录酶都是一条多肽链。鸟类RNA病毒的反转录酶则由两上亚基结构。真核生物中也都分离出具有不同结构的反转录酶。

