Overview:
VEGF-C is a member of the PDGF/VEGF family which is active in angiogenesis and endothelial cell growth, and can also affect the permeability of blood vessels. It is structurally and functionally similar to vascular endothelial growth factor D. VEGFC is an osmosensitive, hypertonicity-driven gene intimately involved in salt-induced hypertension and tonicity-responsive enhancer-binding protein (TONEBP)-VEGFC signaling in mononuclear phagocytes is a major determinant of extracellular volume and blood pressure homeostasis (1).VEGF-C is also essential for embryonic survival and lymphangiogenesis (2).
Gene Aliases:
VEGFC; VRP; Flt4-L
Genbank Number:
NP_005420.1
References:
1. Machnik, A. et.al: Macrophages regulate salt-dependent volume and blood pressure by a vascular endothelial growth factor-C-dependent buffering mechanism. Nature Med. 15: 545-552, 2009.2. Karkkainen, M. J. et.al: Vascular endothelial growth factor C is required for sprouting of the first lymphatic vessels from embryonic veins. Nature Immun. 5: 74-80, 2004.
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我在做5RACE,选用TaKaRa公司的RNA反转录酶(RNaseH-),不知道这个酶好不好,有什么需要注意的。
没有序列识别的功能,他就是简单的催化相邻DNA链的5-P末端和3'-OH末端以磷酸二酯键结合的反应,需ATP作辅酶。 不仅可以催化粘性末端之间或平滑末端之间的DNA的连接,也催化DNA与RNA之间以及少数RNA之间的连接。
反转录酶(Reversetranscripatase)是以RNA为模板指导三磷酸脱氧核苷酸合成互补DNA(cDNA)的酶。哺乳类C型病毒的反转录酶和鼠类B型病毒的反转录酶都是一条多肽链。鸟类RNA病毒的反转录酶则由两上亚基结构。真核生物中也都分离出具有不同结构的反转录酶。

