Overview:
IL-2 or interleukin 2 is a secreted cytokine which referred to as T-cell growth factor, that is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. IL2 can act as a growth hormone that is important for the proliferation of T and B-lymphocytes (1).IL2 expression is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene (2).
Gene Aliases:
TCGF; lymphokine; IL2
Genbank Number:
NP_000577.2
References:
1. Lowenthal, J. W. et.al: Similarities between interleukin-2 receptor number and affinity on activated B and T lymphocytes. Nature 315: 669-672, 1985.2. Hollander, G. A. et.al: Monoallelic expression of the interleukin-2 locus. Science 279: 2118-2121, 1998.
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我在做5RACE,选用TaKaRa公司的RNA反转录酶(RNaseH-),不知道这个酶好不好,有什么需要注意的。
没有序列识别的功能,他就是简单的催化相邻DNA链的5-P末端和3'-OH末端以磷酸二酯键结合的反应,需ATP作辅酶。 不仅可以催化粘性末端之间或平滑末端之间的DNA的连接,也催化DNA与RNA之间以及少数RNA之间的连接。
反转录酶(Reversetranscripatase)是以RNA为模板指导三磷酸脱氧核苷酸合成互补DNA(cDNA)的酶。哺乳类C型病毒的反转录酶和鼠类B型病毒的反转录酶都是一条多肽链。鸟类RNA病毒的反转录酶则由两上亚基结构。真核生物中也都分离出具有不同结构的反转录酶。

