Overview:
Originally identified as a gene induced by IL-9 in mouse T cells and mast cells, IL-22 is a member of the IL-10 family of cytokines. IL-22 inhibits IL-4 production by Th2 cells, and induces acute phase reactants in the pancreas and liver. The IL-22 receptor complex consists of two receptor subunits, IL-22R and IL-10R-beta that belong to the class II cytokine receptor family. Recombinant human IL-22 is a non-disulfide-linked homodimeric protein.
Gene Aliases:
IL-22, IL-TIF, Cytokine Zcyto18, IL-10-related T cell-derived-inducible factor, ILTIF, ZCYTO18Recombinant Human Interleukin-22 (IL22)
Genbank Number:
Q9GZX6
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我在做5RACE,选用TaKaRa公司的RNA反转录酶(RNaseH-),不知道这个酶好不好,有什么需要注意的。
没有序列识别的功能,他就是简单的催化相邻DNA链的5-P末端和3'-OH末端以磷酸二酯键结合的反应,需ATP作辅酶。 不仅可以催化粘性末端之间或平滑末端之间的DNA的连接,也催化DNA与RNA之间以及少数RNA之间的连接。
反转录酶(Reversetranscripatase)是以RNA为模板指导三磷酸脱氧核苷酸合成互补DNA(cDNA)的酶。哺乳类C型病毒的反转录酶和鼠类B型病毒的反转录酶都是一条多肽链。鸟类RNA病毒的反转录酶则由两上亚基结构。真核生物中也都分离出具有不同结构的反转录酶。

