Overview:
PTPRE or protein tyrosine phosphatase receptor type E is a member of the protein tyrosine phosphatase (PTP) family. This family of proteins are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPRE is highly expressed in brain and testis (1). The regulatory roles of this PTP in Ras related signal transduction pathways, cytokines induced signaling, as well as the activation of voltage-gated K+ channels have been identified. PTPRE has a transmembrane segment and a cytoplasmic region containing duplicated PTPase domains (2).
Gene Aliases:
PTPE, P-PTP-epsilon, HPTPE, R-PTP-EPSILON, RP11-380J17.1, DKFZp313F1310, FLJ57799, FLJ58245
Genbank Number:
BC050062
References:
1. Yi, T. et.al: Identification of novel protein tyrosine phosphatases of hematopoietic cells by polymerase chain reaction amplification. Blood 78: 2222-2228, 1991.2. Krueger, N. X. et.al: Structural diversity and evolution of human receptor-like protein tyrosine phosphatases. EMBO J. 9: 3241-3252, 1990.
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我在做5RACE,选用TaKaRa公司的RNA反转录酶(RNaseH-),不知道这个酶好不好,有什么需要注意的。
没有序列识别的功能,他就是简单的催化相邻DNA链的5-P末端和3'-OH末端以磷酸二酯键结合的反应,需ATP作辅酶。 不仅可以催化粘性末端之间或平滑末端之间的DNA的连接,也催化DNA与RNA之间以及少数RNA之间的连接。
反转录酶(Reversetranscripatase)是以RNA为模板指导三磷酸脱氧核苷酸合成互补DNA(cDNA)的酶。哺乳类C型病毒的反转录酶和鼠类B型病毒的反转录酶都是一条多肽链。鸟类RNA病毒的反转录酶则由两上亚基结构。真核生物中也都分离出具有不同结构的反转录酶。

