Recombinant Human His6-Otubain-2 Isoform 1 Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E-554
| Formulation | Xmg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 5 mM DTT |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Otubain-2
Otubain-2 is a C65 type peptidase and a member of the ovarian tumor (OTU) protein super-family found in eukaryotes, viruses and pathogenic bacterium. Otubain-2 associates with the E3 ubiquitin ligases TRAF3 and TRAF6, and has been shown to negatively regulate virus-induced type I IFN induction and the antiviral responses mediated by these ligases. This recombinant protein contains a C-terminal 6-His tag.
- Li S., et al. (2010) J.Biol.Chem. 285: 4291-4297
- Nanao M.H., et al. (2004) EMBO Reports 5: 783-788
FAQs
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我在做5RACE,选用TaKaRa公司的RNA反转录酶(RNaseH-),不知道这个酶好不好,有什么需要注意的。
没有序列识别的功能,他就是简单的催化相邻DNA链的5-P末端和3'-OH末端以磷酸二酯键结合的反应,需ATP作辅酶。 不仅可以催化粘性末端之间或平滑末端之间的DNA的连接,也催化DNA与RNA之间以及少数RNA之间的连接。
反转录酶(Reversetranscripatase)是以RNA为模板指导三磷酸脱氧核苷酸合成互补DNA(cDNA)的酶。哺乳类C型病毒的反转录酶和鼠类B型病毒的反转录酶都是一条多肽链。鸟类RNA病毒的反转录酶则由两上亚基结构。真核生物中也都分离出具有不同结构的反转录酶。

