The BlastR™ filters allows for fast and reliable preparation of genomic DNA (gDNA)-free cell lysate for western blot or immunoprecipitation when used in combination with a denaturing buffer, such as guanidine, urea or high SDS based extraction buffers that usually produce copious viscosity. gDNA contamination is a significant problem with denaturing buffers that can interfere with downstream applications like immunoprecipitation and migration of proteins in SDS-PAGE. Unlike sonication or insulin needle gDNA shearing methods, the BlastR™ filter effectively removes gDNA contamination while having no effect on the integrity of the proteins in the lysate.
- Reduce viscosity of samples
- Rapid isolation of protein extracts
- Preparation of extracts from denaturing lysis buffers
Validation Data: BlastR Rapid Filtration Kit White Paper
Clarification of gDNA from BlastR™ lysate
(A) Viscous sample lysate loaded onto BlastR™ Filter.
(B) Sample passed through Filter system where gDNA is captured. >90% recovery of protein in cell lysate.
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Click on the pdf icon below to download the manual 
For the most recent publications citing this and other Signal-Seeker™ products, see our Signal-Seeker™ Validation Data Page click here
Visit our Signal-Seeker™ Tech Tips and FAQs page for technical tips and frequently asked questions regarding this and other Signal-Seeker™ products click here
If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com
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MicroBCA试剂测定范围是0.5-20μg/ml
楼主自己看看经销商价格
现在跑tricine－sds－page电泳老是跑不好，
我刚做了bradford定量，
觉得差异与空菌对照还是挺明显的，
但是这个方法只能初略的估计，
还是不能精确定量，
做HPLC我现在还没有条件，
所以我想能不能用BCA或者lowry法先进行定量啊？
这种方法如果做出来结果的话，
能不能发个sci之类的文章啊？
希望高手们指点一下！
谢谢了
临床实验室对商品定量试剂盒分析性能的验证.pdf(17431.6k)
至少可以说同一种蛋白定量试剂盒并不适用于所有细胞
蛋白定量试剂盒有分Bradford，BCA，Lowry等，每一种对不同的细胞都有独特的适用范围和灵敏度。所以需要多种蛋白定量试剂盒对比检测才能让结果准确
(1)已包被抗原或抗体的固相载体(免疫吸附剂)；
(2)酶标记的抗原或抗体(结合物)；
(3)酶的底物；
(4)阴性对照品和阳性对照品(定性测定中)，参考标准品和控制血清(定量测定中)；
(5)结合物及标本的稀释液；
(6)洗涤液，在板式ELISA中，常用的稀释液为含0.05%吐温20磷酸缓冲盐水；
(7)酶反应终止液，常用的HRP反应终止液为硫酸，其浓度按加量及比色液的最终体积而异，在板式ELISA中一般采用3mol/L。向左转|向右转
