The BlastR™ filters allows for fast and reliable preparation of genomic DNA (gDNA)-free cell lysate for western blot or immunoprecipitation when used in combination with a denaturing buffer, such as guanidine, urea or high SDS based extraction buffers that usually produce copious viscosity. gDNA contamination is a significant problem with denaturing buffers that can interfere with downstream applications like immunoprecipitation and migration of proteins in SDS-PAGE. Unlike sonication or insulin needle gDNA shearing methods, the BlastR™ filter effectively removes gDNA contamination while having no effect on the integrity of the proteins in the lysate.
- Reduce viscosity of samples
- Rapid isolation of protein extracts
- Preparation of extracts from denaturing lysis buffers
Validation Data: BlastR Rapid Filtration Kit White Paper
Clarification of gDNA from BlastR™ lysate
(A) Viscous sample lysate loaded onto BlastR™ Filter.
(B) Sample passed through Filter system where gDNA is captured. >90% recovery of protein in cell lysate.
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Click on the pdf icon below to download the manual 
For the most recent publications citing this and other Signal-Seeker™ products, see our Signal-Seeker™ Validation Data Page click here
Visit our Signal-Seeker™ Tech Tips and FAQs page for technical tips and frequently asked questions regarding this and other Signal-Seeker™ products click here
If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com
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试剂盒里有详细的说明书，告诉你样品需要多少量，每个试剂需要加入多少量，和详细的实验步骤，一般买来就可以用，不用人教。
所以你问一个样需要多少量是没法回答的，测定过程是要加很多种试剂的。
1.模板提取（一般为RNA）：Trizol、氯仿、异丙醇、无水乙醇、DEPC处理水
2.模板浓度测定：分光光度计或NanoDrop
3.逆转录：逆转录试剂盒（或者一步法试剂盒），这一步可以用普通PCR做，也可以用水域做。
4.荧光定量PCR试剂：通常有用SYBR Green Mix做的，但是这里建议你用EvaGreen做，灵敏度和平行性都要好于SYBR Green，并且如果你那是ABI或者Stratagene的PCR如果用SYBR Green还需要加一步Rox很麻烦。
5.其他：除了以上的那些还需要离心管、PCR管或板（Axygen反应比较好）、移液枪等，暂时就想到这么多。
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EliKine™ 小鼠 血管内皮生长因子 ELISA定量试剂盒
EliKine™ 大鼠 干扰素-γ ELISA定量试剂盒
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只要引物设计的可以在基因组上做，那么理论上是没问题的！
只需要用RT-PCR荧光定量试剂盒里面的第二个，专门用来做Real-timePCR的那些试剂就可以了！
