If you thought intubating small rodents was too difficult, the BioLITE Intubation Illumination System is for you. The BioLITE system provides better anatomical visualization and combines several tools into one reducing the intubation time making the intubation process fast and easy.
The BioLITE System consists of a family of products: Fiber Optic Illuminator (FO-Light), Fiber Optic Stylets (FOS), Endotracheal Tubes (ETT), and Lung Inflation Bulbs (LIB) packaged in a variety of kits to meet the individual needs of a variety of research interests.
Unlike other flashlight based fiber optic illuminators, the FO-Light houses a patent pending optical system that provides highly efficient light coupling into small core fiber optics resulting in intense illumination. Without the optical system, the intensity of the light output is insufficient to visualize anatomical features, which is precisely the reason why other flashlight based fiber optic illuminators are not compatible with small core fiber optics.
By using a small core fiber optic as both the stylet and guide wire, the FOS provides enough rigidity for applying mechanical force while remaining flexible enough to traverse convoluted paths. Also since light can be delivered through the stylet/guide wire, you are able to illuminate the discrete places in the oropharynx directly where you need it.
As accessories to the illuminator and stylets, the endotracheal tubes and lung inflation bulbs have specific qualities and provide feedback that make them essential to any intubation procedure. The Endotracheal Tubes are urethane based IV catheters that are much softer and more flexible than the PTFE based catheters which minimizes the trauma associated with the intubation. The lung inflation bulbs provide a means for verifying correct intubation through direct visualization of chest distention, which is more reliable than observing mirror fogging or abdominal breathing.The BioLITE System is available in the following kits: a Large Rat Kit (200+ grams, LR-Kit), a Small Rat Kit (less than 200 grams, SR-Kit), and a Mouse Kit (10+ grams, MI-Kit). Each kit contains a fiber optic illuminator (FO-Light), the appropriate size fiber optic stylet (FOS-xxx), a 5 pack of the appropriate size endotracheal tubes (ETT-xx-05), and a 3 pack of lung inflation bulbs (LIB-03), all the necessary equipment to perform small rodent intubations fast, easy, and relatively painless.
The system is also offered in a Complete Kit (CO-Kit) that includes all the BioLITE System products for the mouse and both sizes of rat.
Though the BioLITE System was initially created for intubation, it remains a highly efficient small core fiber optic illumination system that can essentially be used in any application that requires intense illumination of a small space in a tortuous location. For those interested in alternate applications or have no need for the intubation accessories, a Stylets Kit (FO-Kit) that includes a mouse fiber optic stylet (FOS-400, 400µm core diameter), a small rat fiber optic stylet (FOS-600), a big rat fiber optic stylet (FOS-800), and a fiber optic illuminator (FO-Light).
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试剂盒里有详细的说明书，告诉你样品需要多少量，每个试剂需要加入多少量，和详细的实验步骤，一般买来就可以用，不用人教。
所以你问一个样需要多少量是没法回答的，测定过程是要加很多种试剂的。
1.模板提取（一般为RNA）：Trizol、氯仿、异丙醇、无水乙醇、DEPC处理水
2.模板浓度测定：分光光度计或NanoDrop
3.逆转录：逆转录试剂盒（或者一步法试剂盒），这一步可以用普通PCR做，也可以用水域做。
4.荧光定量PCR试剂：通常有用SYBR Green Mix做的，但是这里建议你用EvaGreen做，灵敏度和平行性都要好于SYBR Green，并且如果你那是ABI或者Stratagene的PCR如果用SYBR Green还需要加一步Rox很麻烦。
5.其他：除了以上的那些还需要离心管、PCR管或板（Axygen反应比较好）、移液枪等，暂时就想到这么多。
EliKine™ 人 肝细胞生长因子 ELISA定量试剂盒
EliKine™ 人 干扰素-α ELISA定量试剂盒
EliKine™ 人 干扰素-γ ELISA定量试剂盒
EliKine™ 人 白介素-1α ELISA定量试剂盒
EliKine™ 小鼠 白介素-22 ELISA定量试剂盒
EliKine™ 小鼠 转化生长因子-β1 ELISA定量试剂盒
EliKine™ 小鼠 肿瘤坏死因子-α ELISA定量试剂盒
EliKine™ 小鼠 血管内皮生长因子 ELISA定量试剂盒
EliKine™ 大鼠 干扰素-γ ELISA定量试剂盒
EliKine™ 大鼠 白介素-1β ELISA定量试剂盒
EliKine™ 人 促甲状腺激素 ELISA定量试剂盒
只要引物设计的可以在基因组上做，那么理论上是没问题的！
只需要用RT-PCR荧光定量试剂盒里面的第二个，专门用来做Real-timePCR的那些试剂就可以了！
