

Highlights
- Quick recovery of ultra-pure DNA from agarose gels.
- Column design permits DNA elution at high concentrations into minimal volumes.
- Eluted DNA is well-suited for use in DNA ligation, sequencing, labeling, PCR, etc.
Description
Applicable For | Next Generation sequencing, ligation reactions, PCR, labeling, and restriction endonuclease digestions. |
---|---|
Elution Volume | ≥ 6 µl of DNA Elution Buffer |
Equipment | Microcentrifuge |
Purity | A260/A280 >1.8, A260/A230>1.8 |
Sample Source | DNA excised from TAE/TBE-buffered agarose gels. |
Size Range | 50 bp to 23 kb |
Yield | Typically, up to 5 µg total DNA per column can be eluted with ≥6 µl water. For DNA 50 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70% |
Q1: What is the difference between capped and uncapped?
The only difference is the cap. Everything else between the columns (max capacity, column matrix, etc.) is the same. Unsure which to use? It mainly comes down to preference. Some people like the capped columns for easy labeling or added security while others like the uncapped columns for faster sample processing.
Q2: Is it possible to use higher binding capacity columns for the Zymoclean workflow?
Zymoclean has been validated for use with Zymo-Spin IIC and IIN columns. Other columns with higher binding capacity have not been validated by Zymo Research but have successfully been used by users.
Q3: Are the columns and DNA Wash buffer interchangeable with the DCC-5 Kit?
Yes. Zymo-Spin I/IC Columns and DNA Wash Buffer are interchangeable.
Q4: Should more Agarose Dissolving Buffer be used for higher % gels?
Add 4 volumes of Agarose Dissolving Buffer for agarose gels >2% and ensure the gel slice has been fully dissolved.
Q5: Can Zymoclean be used for samples in solution (without agarose gel)?
No, high efficiency recovery is not guaranteed and the success of the purification can be variable.
Q6: Can Zymoclean be used to recover ssDNA?
If only isolating ssDNA, Zymo Research recommends the Zymoclean Gel RNA Recovery Kit. With some modification of the Zymoclean Gel DNA Recovery protocol, it is possible to recover ssDNA: After gel dissolving (step 3), add half a volume of isopropanol. Continue with the protocol.
Q7: What is the maximum weight of gel slice that can be used?
Up to 400 mg of agarose gel.
“The best advantage about this kit is that it is really quick to make it, and the total DNA recuperated have a good quality and it have a good concentration to follow the experiments.”
-Ingrid M.
“The kit was very easy to follow and yielded good results. It was more affordable than the Qiagen Gel Extraction kits, but worked just as efficiently. Solid product“
-Thomas B (University of Tennessee, Knoxville)
“Protocols are very well written and Instructions easy to follow. Always had good results with your products.”
-Simin H. (University of California, Irvine)
Read MoreCat # | Name | Size | Price | |
---|---|---|---|---|
C1001-50 | Collection Tubes | 50 Pack | $15.00 | |
C1004-50 | Zymo-Spin IC Columns | 50 Pack | $53.00 | |
C1003-50 | Zymo-Spin I Columns | 50 Pack | $50.00 | |
D3004-4-4 | DNA Elution Buffer | 4 ml | $10.00 | |
D3004-4-1 | DNA Elution Buffer | 1 ml | $11.00 | |
D4003-2-6 | DNA Wash Buffer (Concentrate) | 6 ml | $10.00 | |
D4001-1-100 | ADB (Agarose Dissolving Buffer) | 100 ml | $63.00 | |
D4001-1-50 | ADB (Agarose Dissolving Buffer) | 50 ml | $33.00 | |
D4003-2-24 | DNA Wash Buffer (Concentrate) | 24 ml | $33.00 |
ebiomall.com






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临床实验室对商品定量试剂盒分析性能的验证.pdf(17431.6k)
两个意义不一样啊,定性是确定你抗体是阴还是阳的,如果阳证明2个情况,一个是你感染了丙肝病毒,另一个是你曾经感染过这个病毒,现在已经好了。但是到底是哪个情况还要进一步做定量测试既病毒RNA检测。如果这个测试值在最低线以下就证明你现在没事,不具有传染性也不是患者只是携带者;如果高于最低线那你就是患者了,就需要治疗
MicroBCA试剂测定范围是0.5-20μg/ml
楼主自己看看经销商价格
只要引物设计的可以在基因组上做,那么理论上是没问题的!
只需要用RT-PCR荧光定量试剂盒里面的第二个,专门用来做Real-timePCR的那些试剂就可以了!
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1.模板提取(一般为RNA):Trizol、氯仿、异丙醇、无水乙醇、DEPC处理水
2.模板浓度测定:分光光度计或NanoDrop
3.逆转录:逆转录试剂盒(或者一步法试剂盒),这一步可以用普通PCR做,也可以用水域做。
4.荧光定量PCR试剂:通常有用SYBR Green Mix做的,但是这里建议你用EvaGreen做,灵敏度和平行性都要好于SYBR Green,并且如果你那是ABI或者Stratagene的PCR如果用SYBR Green还需要加一步Rox很麻烦。
5.其他:除了以上的那些还需要离心管、PCR管或板(Axygen反应比较好)、移液枪等,暂时就想到这么多。

