Highlights
- Quick recovery of ultra-pure DNA from agarose gels.
- Column design permits DNA elution at high concentrations into minimal volumes.
- Eluted DNA is well-suited for use in DNA ligation, sequencing, labeling, PCR, etc.
Description
| Applicable For | Next Generation sequencing, ligation reactions, PCR, labeling, and restriction endonuclease digestions. |
|---|---|
| Elution Volume | ≥ 6 µl of DNA Elution Buffer |
| Equipment | Microcentrifuge |
| Purity | A260/A280 >1.8, A260/A230>1.8 |
| Sample Source | DNA excised from TAE/TBE-buffered agarose gels. |
| Size Range | 50 bp to 23 kb |
| Yield | Typically, up to 5 µg total DNA per column can be eluted with ≥6 µl water. For DNA 50 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70% |
Q1: What is the difference between capped and uncapped?
The only difference is the cap. Everything else between the columns (max capacity, column matrix, etc.) is the same. Unsure which to use? It mainly comes down to preference. Some people like the capped columns for easy labeling or added security while others like the uncapped columns for faster sample processing.
Q2: Is it possible to use higher binding capacity columns for the Zymoclean workflow?
Zymoclean has been validated for use with Zymo-Spin IIC and IIN columns. Other columns with higher binding capacity have not been validated by Zymo Research but have successfully been used by users.
Q3: Are the columns and DNA Wash buffer interchangeable with the DCC-5 Kit?
Yes. Zymo-Spin I/IC Columns and DNA Wash Buffer are interchangeable.
Q4: Should more Agarose Dissolving Buffer be used for higher % gels?
Add 4 volumes of Agarose Dissolving Buffer for agarose gels >2% and ensure the gel slice has been fully dissolved.
Q5: Can Zymoclean be used for samples in solution (without agarose gel)?
No, high efficiency recovery is not guaranteed and the success of the purification can be variable.
Q6: Can Zymoclean be used to recover ssDNA?
If only isolating ssDNA, Zymo Research recommends the Zymoclean Gel RNA Recovery Kit. With some modification of the Zymoclean Gel DNA Recovery protocol, it is possible to recover ssDNA: After gel dissolving (step 3), add half a volume of isopropanol. Continue with the protocol.
Q7: What is the maximum weight of gel slice that can be used?
Up to 400 mg of agarose gel.
“The best advantage about this kit is that it is really quick to make it, and the total DNA recuperated have a good quality and it have a good concentration to follow the experiments.”
-Ingrid M.
“The kit was very easy to follow and yielded good results. It was more affordable than the Qiagen Gel Extraction kits, but worked just as efficiently. Solid product“
-Thomas B (University of Tennessee, Knoxville)
“Protocols are very well written and Instructions easy to follow. Always had good results with your products.”
-Simin H. (University of California, Irvine)
Read More| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| C1001-50 | Collection Tubes | 50 Pack | $15.00 | |
| C1004-50 | Zymo-Spin IC Columns | 50 Pack | $53.00 | |
| C1003-50 | Zymo-Spin I Columns | 50 Pack | $50.00 | |
| D3004-4-4 | DNA Elution Buffer | 4 ml | $10.00 | |
| D3004-4-1 | DNA Elution Buffer | 1 ml | $11.00 | |
| D4003-2-6 | DNA Wash Buffer (Concentrate) | 6 ml | $10.00 | |
| D4001-1-100 | ADB (Agarose Dissolving Buffer) | 100 ml | $63.00 | |
| D4001-1-50 | ADB (Agarose Dissolving Buffer) | 50 ml | $33.00 | |
| D4003-2-24 | DNA Wash Buffer (Concentrate) | 24 ml | $33.00 |
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如果要是直接注射,就不知道了
现在跑tricine-sds-page电泳老是跑不好,
我刚做了bradford定量,
觉得差异与空菌对照还是挺明显的,
但是这个方法只能初略的估计,
还是不能精确定量,
做HPLC我现在还没有条件,
所以我想能不能用BCA或者lowry法先进行定量啊?
这种方法如果做出来结果的话,
能不能发个sci之类的文章啊?
希望高手们指点一下!
谢谢了
3.逆转录:逆转录试剂盒(或者一步法试剂盒),这一步可以用普通PCR做,也可以用水域做。
4.荧光定量PCR试剂:通常有用SYBR Green Mix做的,但是这里建议你用EvaGreen做,灵敏度和平行性都要好于SYBR Green,并且如果你那是ABI或者Stratagene的PCR如果用SYBR Green还需要加一步Rox很麻烦。
5.其他:除了以上的那些还需要离心管、PCR管或板(Axygen反应比较好)、移液枪等,暂时就想到这么多。
加入荧光标记探针,巧妙地把核算扩增、杂交、光谱分析和实时检测技术结合在一起,借助于荧光信号来检测PCR产物。一方面提高了灵敏度,另一方面还可以做到PCR每循环一次就收集一个数据,建立实时扩增曲线,准确地确定CT值,从而根据CT值确定起始DNA的拷贝数,做到真正意义上的DNA定量。另外由于CT值是一个完全客观的参数,CT值越小,模版DNA的起始拷贝数越小。因此,利用CT值确定DNA拷贝数实时PCR方法比普通终点定量方法更加准确

