| Fluorouracil (Adrucil)Antitumor agent;inhibitor of thymidylate synthase |
Sample solution is provided at 25 µL, 10mM.
Quality Control & MSDS
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- Purity = 99.86%
- COA (Certificate Of Analysis)
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- NMR (Nuclear Magnetic Resonance)
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Chemical structure
Related Biological Data
Related Biological Data
Related Biological Data
| Cell experiment [1]: | |
Cell lines | Human colon carcinoma cell line HT-29 |
Preparation method | The solubility of this compound in DMSO is >10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months. |
Reaction Conditions | 0.01 ~ 10 μM; 7 days |
Applications | In human colon carcinoma cell line HT-29, Fluorouracil suppressed cell viability, with the IC50 value of 2.5 μM. |
| Animal experiment [2]: | |
Animal models | C56B1/6 mice bearing Colon 38 tumor |
Dosage form | 100 mg/kg; i.p.; weekly |
Applications | Fluorouracil (100mg/kg) significantly suppressed tumor growth of murine colon carcinoma Colon 38 with tumor-doubling time (TD), growth-delay factor (GDF), and T/C of 26.5 days, 4.4, and 14%. |
Other notes | Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
References: [1]. Schwartz EL, Baptiste N, Wadler S, Makower D. Thymidine phosphorylase mediates the sensitivity of human colon carcinoma cells to 5-fluorouracil. J Biol Chem. 1995 Aug 11;270(32):19073-7. [2]. Van Laar JA, Rustum YM, Van der Wilt CL, Smid K, Kuiper CM, Pinedo HM, Peters GJ. Tumor size and origin determine the antitumor activity of cisplatin or 5-fluorouracil and its modulation by leucovorin in murine colon carcinomas. Cancer Chemother Pharmacol. 1996;39(1-2):79-89. | |
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| Cas No. | 51-21-8 | SDF | Download SDF |
| Synonyms | 5-Fluorouracil,5-FU, Efudex, Adrucil, Carac | ||
| Chemical Name | 5-fluoro-1H-pyrimidine-2,4-dione | ||
| Canonical SMILES | C1=C(C(=O)NC(=O)N1)F | ||
| Formula | C4H3FN2O2 | M.Wt | 130.1 |
| Solubility | ≥6.5 mg/mL in DMSO, <2.51 mg/ml="" in="" etoh,="" ≥10.04="" mg/ml="" in="" h2o="" with="" ultrasonic="" and="" warming=""> | Storage | Store at -20°C |
| Physical Appearance | A solid | Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
Fluorouracil (Adrucil), a heterocyclic aromatic organic compound, is a potent anticancer agent widely used for the treatment of solid tumors, including breast cancer, ovarian cancer, head and neck cancer, and colon cancer. As an analogue of uracil, fluorouracil has a fluorine atom replacing the hydrogen atom at the C-5 position. Due to its similar chemical structure to DNA and RNA, fluorouracil and metabolites exert strong anticancer activities through incorporation into DNA and RNA and inhibition of thymidylate synthase (TS). Fluorouracil is metabolized into fluorodeoxyuridine monophosphate (FdUMP), which inhibits TS by forming a stable complex with it and subsequently suppresses the production of deoxythymidine monophosphate (dTMP), an essential enzyme involved in DNA replication and repair, leading to cytotoxicity and cell death.
Reference
Ning Zhang, Ying Yin, Shen-Jie Xu and Wei-Shan Chen. 5-Fluorouracil: mechanisms of resistance and reversal strategies. Molecules 2008, 13, 1551-1569
Michael D. Wyatt and David M. Wilson III. Participation of DNA repair in the response to 5-fluorouracil. Cell Mol Life Sci. 2009; 66(5): 788-799
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LOQ-定量限LOD-检测限
1.模板提取（一般为RNA）：Trizol、氯仿、异丙醇、无水乙醇、DEPC处理水
2.模板浓度测定：分光光度计或NanoDrop
3.逆转录：逆转录试剂盒（或者一步法试剂盒），这一步可以用普通PCR做，也可以用水域做。
4.荧光定量PCR试剂：通常有用SYBR Green Mix做的，但是这里建议你用EvaGreen做，灵敏度和平行性都要好于SYBR Green，并且如果你那是ABI或者Stratagene的PCR如果用SYBR Green还需要加一步Rox很麻烦。
5.其他：除了以上的那些还需要离心管、PCR管或板（Axygen反应比较好）、移液枪等，暂时就想到这么多。
1.步骤简单，45分钟内完成测定，比经典Lowry法快4倍而且更加方便。
2.灵敏度高，检测浓度下限达到25μg/ml，最小检测蛋白量达到0.5μg，待测样品体积为1-20μl 。
3.BCA法测定蛋白浓度不受绝大部分样品中的去污剂等化学物质的影响，可以兼容样品中高达5%的SDS，5%的Triton X-100，5%的Tween 20，60，80。
4.在20-2000μg/ml浓度范围内有良好的线性关系。
5.检测不同蛋白质分子的变异系数远小于考马斯亮蓝法蛋白定量。
临床实验室对商品定量试剂盒分析性能的验证.pdf(17431.6k)
加入荧光标记探针，巧妙地把核算扩增、杂交、光谱分析和实时检测技术结合在一起，借助于荧光信号来检测PCR产物。一方面提高了灵敏度，另一方面还可以做到PCR每循环一次就收集一个数据，建立实时扩增曲线，准确地确定CT值，从而根据CT值确定起始DNA的拷贝数，做到真正意义上的DNA定量。另外由于CT值是一个完全客观的参数，CT值越小，模版DNA的起始拷贝数越小。因此，利用CT值确定DNA拷贝数实时PCR方法比普通终点定量方法更加准确
