| Catalog number | AN033G |
| Product type | ELISA |
| Quantity | 96 Tests (12x8 breakable strip wells) |
| Sample volume | 10 µl/well |
| Species | Human |
| Storage and Stability | Product should be stored at 2-8 °C. |
| Precautions | For research use only. Not for use in diagnostic procedures. |
| References | 1. Emlen W; O"Neill L Clinical significance of antinuclear antibodies: comparison of detection with immunofluorescence and enzyme-linked immunosorbent assays. Arthritis Rheum 1997;40(9):1612-8. 2. Gonz´alez C; Martin T; Arroyo T; Garc´ia-Isidoro M; Navajo JA; Gonz´alez-Buitrago JM. Comparison and variation of different methodologies for the detection of autoantibodies to nuclear antigens (ANA). J Clin Lab Anal 1997;11(6):388-92.3. Parveen S; Morshed SA; Nishioka M. High prevalence of antibodies to recombinant CENP-B in primary biliary cirrhosis: nuclear immunofluorescence patterns and ELISA reactivities. J Gastroenterol Hepatol 1995;10(4):438-45.4. Welin Henriksson E; Hansson H; Karlsson-Parra A; Pettersson I. Autoantibody profiles in canine ANA-positive sera investigated by immunoblot and ELISA. Vet Immunol Immunopathol 1998;61(2-4):157-70.5. Koh WH; Dunphy J; Whyte J; Dixey J; McHugh NJ. Characterisation of anticytoplasmic antibodies and their clinical associations [see comments]. Ann Rheum Dis 1995;54(4):269-73.6. Spronk PE; Bootsma H; Horst G; Huitema MG; Limburg PC; Cohen Tervaert JW; Kallenberg CG. Antineutrophil cytoplasmic antibodies in systemic lupus erythematosus. Br J Rheumatol 1996;35(7):625-31. |
ANA-Screen-IgG-ELISA-Package-InsertType : PDF
dsDNA IgG ELISA

SSA IgG ELISA

SSB IgG ELISA

Sm IgG ELISA

RNP/Sm IgG ELISA

Scl-70 IgG ELISA

ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
LOQ-定量限LOD-检测限
临床实验室对商品定量试剂盒分析性能的验证.pdf(17431.6k)
1.模板提取(一般为RNA):Trizol、氯仿、异丙醇、无水乙醇、DEPC处理水
2.模板浓度测定:分光光度计或NanoDrop
3.逆转录:逆转录试剂盒(或者一步法试剂盒),这一步可以用普通PCR做,也可以用水域做。
4.荧光定量PCR试剂:通常有用SYBR Green Mix做的,但是这里建议你用EvaGreen做,灵敏度和平行性都要好于SYBR Green,并且如果你那是ABI或者Stratagene的PCR如果用SYBR Green还需要加一步Rox很麻烦。
5.其他:除了以上的那些还需要离心管、PCR管或板(Axygen反应比较好)、移液枪等,暂时就想到这么多。
至少可以说同一种蛋白定量试剂盒并不适用于所有细胞
蛋白定量试剂盒有分Bradford,BCA,Lowry等,每一种对不同的细胞都有独特的适用范围和灵敏度。所以需要多种蛋白定量试剂盒对比检测才能让结果准确
1.模板提取(一般为RNA):Trizol、氯仿、异丙醇、无水乙醇、DEPC处理水
2.模板浓度测定:分光光度计或NanoDrop
3.逆转录:逆转录试剂盒(或者一步法试剂盒),这一步可以用普通PCR做,也可以用水域做。
4.荧光定量PCR试剂:通常有用SYBR Green Mix做的,但是这里建议你用EvaGreen做,灵敏度和平行性都要好于SYBR Green,并且如果你那是ABI或者Stratagene的PCR如果用SYBR Green还需要加一步Rox很麻烦。
5.其他:除了以上的那些还需要离心管、PCR管或板(Axygen反应比较好)、移液枪等,暂时就想到这么多。
如果要是直接注射,就不知道了

