| Catalog number | CM028M |
| Product type | ELISA |
| Quantity | 96 Tests (12x8 breakable strip wells) |
| Sample volume | 10 µl/well |
| Species | Human |
| Storage and Stability | Product should be stored at 2-8 °C. |
| Precautions | For research use only. Not for use in diagnostic procedures. |
| References | 1.Mangano WE; Gruninger RP. Use of viral cultures and serologic tests for cytomegalovirus infection. Rational or random? Am J Clin Pathol 1996;106(2):180-4. 2.Gupta CK; Leszczynski J; Gupta RK; Siber GR. An enzyme immunoassay based micro-neutralization test for titration of antibodies to human cytomegalovirus (CMV) and its correlation with direct ELISA measuring CMV IgG antibodies. Biologicals 1996;24(1):41-9. 3.Guti´errez J; Maroto MC. A comparison of two ELISA methods for the investigation of anti-cytomegalovirus IgG antibodies. Microbios 1997;90(364-365):151-4. 4.Gratacap-Cavallier B; Bosson JL; Morand P; Dutertre N; Chanzy B; Jouk PS; Vandekerckhove C; Cart-Lamy P; Seigneurin JM. Cytomegalovirus seroprevalence in French pregnant women: parity and place of birth as major predictive factors. Eur J Epidemiol 1998;14(2):147-52. 5.Schmidt CA; Oettle H; Peng R; Neuhaus P; Blumhardt G; Lohmann R; Wilborn F; Osthoff K; Oertel J; Timm H; et al. Comparison of polymerase chain reaction from plasma and buffy coat with antigen detection and occurrence of immunoglobulin M for the demonstration of cytomegalovirus infection after liver transplantation. Transplantation 1995;59(8):1133-8. 6.Rosenthal SL; Stanberry LR; Biro FM; Slaoui M; Francotte M; Koutsoukos M; Hayes M; Bernstein DI. Seroprevalence of herpes simplex virus types 1 and 2 and cytomegalovirus in adolescents. Clin Infect Dis 1997;24(2):135-9. |
CMV-IgM-ELISA-Package-InsertType : PDF
CMV IgG ELISA

ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
试剂盒里有详细的说明书,告诉你样品需要多少量,每个试剂需要加入多少量,和详细的实验步骤,一般买来就可以用,不用人教。
所以你问一个样需要多少量是没法回答的,测定过程是要加很多种试剂的。
1.模板提取(一般为RNA):Trizol、氯仿、异丙醇、无水乙醇、DEPC处理水
2.模板浓度测定:分光光度计或NanoDrop
3.逆转录:逆转录试剂盒(或者一步法试剂盒),这一步可以用普通PCR做,也可以用水域做。
4.荧光定量PCR试剂:通常有用SYBR Green Mix做的,但是这里建议你用EvaGreen做,灵敏度和平行性都要好于SYBR Green,并且如果你那是ABI或者Stratagene的PCR如果用SYBR Green还需要加一步Rox很麻烦。
5.其他:除了以上的那些还需要离心管、PCR管或板(Axygen反应比较好)、移液枪等,暂时就想到这么多。
EliKine™ 人 肝细胞生长因子 ELISA定量试剂盒
EliKine™ 人 干扰素-α ELISA定量试剂盒
EliKine™ 人 干扰素-γ ELISA定量试剂盒
EliKine™ 人 白介素-1α ELISA定量试剂盒
EliKine™ 小鼠 白介素-22 ELISA定量试剂盒
EliKine™ 小鼠 转化生长因子-β1 ELISA定量试剂盒
EliKine™ 小鼠 肿瘤坏死因子-α ELISA定量试剂盒
EliKine™ 小鼠 血管内皮生长因子 ELISA定量试剂盒
EliKine™ 大鼠 干扰素-γ ELISA定量试剂盒
EliKine™ 大鼠 白介素-1β ELISA定量试剂盒
EliKine™ 人 促甲状腺激素 ELISA定量试剂盒
只要引物设计的可以在基因组上做,那么理论上是没问题的!
只需要用RT-PCR荧光定量试剂盒里面的第二个,专门用来做Real-timePCR的那些试剂就可以了!

