| Catalog number | LH231F |
| Product type | ELISA |
| Quantity | 96 Tests (12x8 breakable strip wells) |
| Standard range | 3.1-50 mIU/ml |
| Analytical Sensitivity | 3.1 mIU/ml |
| Sample volume | 50 µl/well |
| Species | Human |
| Storage and Stability | Product should be stored at 2-8 °C. |
| Precautions | For research use only. Not for use in diagnostic procedures. |
| References | 1. Frank JE; Faix JE; Hermos RJ; Mullaney DM; Rojan DA; Mitchell ML; Klein RZ Thyroid function in very low birth weight infants: effects on neonatal hypothyroidism screening. J Pediatr 1996;128(4):548-54. 2. Thakur C; Saikia TC; Yadav RN. Total serum levels of triiodothyronine (T3) thyroxine (T4) and thyrotropine (LH) in school going children of Dibrugarh district: an endemic goitre region of Assam. Indian J Physiol Pharmacol 1997;41(2):167-70. 3. Morimoto K; Inouye K.A sensitive enzyme immunoassay of human thyroid-stimulating hormone (LH) using bispecific F(ab")2 fragments recognizing polymerized alkaline phosphatase and LH. J Immunol Methods 1997;205(1):81-90. 4. Maes M; Mommen K; Hendrickx D; Peeters D; D"Hondt P; Ranjan R; De Meyer F; Scharp´e S. Components of biological variation, including seasonality, in blood concentrations of LH, TT3, FT4, PRL, cortisol and testosterone in healthy volunteers. Clin Endocrinol (Oxf) 1997;46(5):587-98. |
LH ELISA Package InsertType : PDF
LH lumELISA
FSH ELISA

FSH lumELISA
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现在跑tricine-sds-page电泳老是跑不好,
我刚做了bradford定量,
觉得差异与空菌对照还是挺明显的,
但是这个方法只能初略的估计,
还是不能精确定量,
做HPLC我现在还没有条件,
所以我想能不能用BCA或者lowry法先进行定量啊?
这种方法如果做出来结果的话,
能不能发个sci之类的文章啊?
希望高手们指点一下!
谢谢了
1.步骤简单,45分钟内完成测定,比经典Lowry法快4倍而且更加方便。
2.灵敏度高,检测浓度下限达到25μg/ml,最小检测蛋白量达到0.5μg,待测样品体积为1-20μl 。
3.BCA法测定蛋白浓度不受绝大部分样品中的去污剂等化学物质的影响,可以兼容样品中高达5%的SDS,5%的Triton X-100,5%的Tween 20,60,80。
4.在20-2000μg/ml浓度范围内有良好的线性关系。
5.检测不同蛋白质分子的变异系数远小于考马斯亮蓝法蛋白定量。
谢谢谢

