| Catalog number | MP021M |
| Product type | ELISA |
| Quantity | 96 Tests (12x8 breakable strip wells) |
| Sample volume | 10 µl/well |
| Species | Human |
| Storage and Stability | Product should be stored at 2-8 °C. |
| Precautions | For research use only. Not for use in diagnostic procedures. |
| References | 1. Quinn TC. Diagnosis of atypical pneumonias: Legionella, Chlamydia, and Mycoplasma infections. Ann Intern Med 1996;124:591-4. 2. Cimolai N, Cheong ACH. An assessment of a new diagnostic indirect enzyme immunoassay for the detection of anti-Mycoplasma pneumoniae IgM. Am J Clin Pathol 1996;105:205-9. 3. 5. Shearman MJ, Cubie HA, Inglis JM. Mycoplasma pneumoniae infection: early diagnosis by detection of specific IgM by immunofluorescence. Br J Biomed Sci 1993;50:305-8. 4. Lee SH, Charoenying S, Brennan T, Markowski M, Mayo DR. Comparative studies of three serologic methods for the measurement of Mycoplasma pneumoniae antibodies. Am J Clin Pathol 1989;92:342-7. 5. Kenny GE, Kaiser GG, Cooney MK, Foy HM. Diagnosis of Mycoplasma pneumoniae pneumonia: sensitivities and specificities of serology with lipid antigen and isolation of the organism on soy peptone medium for identification of infections. J Clin Microbiol 1990;28:2087- 93. 6. Aubert G, Pozzetto B, Gaudin OG, Hafid J, Mbida AD, Ros A. Evaluation of five commercial tests: complement fixation, microparticle agglutination, indirect immunofluorescence, enzyme-linked immunosorbent assay and latex agglutination, in comparison to immunoblotting for Mycoplasma pneumoniae serology. Ann Biol Clin 1992;50:593 7. Kok T, Mickan LD, Burrell CJ. Routine diagnosis of seven respiratory viruses and Mycoplasma pneumoniae by enzyme immunoassay. J Virol Methods 1994;50:87-100. 8. Kleemola M, Räty R, Karjalainen J, Schuy W, Gerstenecker B, Jacobs E. Evaluation of an antigen-capture enzyme immunoassay for rapid diagnosis of Mycoplasma pneumoniae infection. Eur J Clin Microbiol Infect Dis 1993;12:872-5. |
Mycoplasma IgM ELISA Package InsertType : PDF
Mycoplasma IgG ELISA
ebiomall.com
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LOQ-定量限LOD-检测限
临床实验室对商品定量试剂盒分析性能的验证.pdf(17431.6k)
1.模板提取（一般为RNA）：Trizol、氯仿、异丙醇、无水乙醇、DEPC处理水
2.模板浓度测定：分光光度计或NanoDrop
3.逆转录：逆转录试剂盒（或者一步法试剂盒），这一步可以用普通PCR做，也可以用水域做。
4.荧光定量PCR试剂：通常有用SYBR Green Mix做的，但是这里建议你用EvaGreen做，灵敏度和平行性都要好于SYBR Green，并且如果你那是ABI或者Stratagene的PCR如果用SYBR Green还需要加一步Rox很麻烦。
5.其他：除了以上的那些还需要离心管、PCR管或板（Axygen反应比较好）、移液枪等，暂时就想到这么多。
至少可以说同一种蛋白定量试剂盒并不适用于所有细胞
蛋白定量试剂盒有分Bradford，BCA，Lowry等，每一种对不同的细胞都有独特的适用范围和灵敏度。所以需要多种蛋白定量试剂盒对比检测才能让结果准确
1.模板提取（一般为RNA）：Trizol、氯仿、异丙醇、无水乙醇、DEPC处理水
2.模板浓度测定：分光光度计或NanoDrop
3.逆转录：逆转录试剂盒（或者一步法试剂盒），这一步可以用普通PCR做，也可以用水域做。
4.荧光定量PCR试剂：通常有用SYBR Green Mix做的，但是这里建议你用EvaGreen做，灵敏度和平行性都要好于SYBR Green，并且如果你那是ABI或者Stratagene的PCR如果用SYBR Green还需要加一步Rox很麻烦。
5.其他：除了以上的那些还需要离心管、PCR管或板（Axygen反应比较好）、移液枪等，暂时就想到这么多。
如果要是直接注射，就不知道了
