Description
Product Highlights
- Fast - streamlined protocol for simple recovery of PCR products in 10 minutes and DNA from gel slices in 20 minutes
- Efficient - column matrix developed for recovery rates of between 70% and 95%
- High-performance - extraction of consistently high-quality DNA fragments, ideal for use in all downstream applications
- Versatile - single buffer promotes effective DNA recovery from both TAE and TBE agarose gels
- Safe - no hazardous phenol/chloroform extraction
Product Description
ISOLATE II PCR and Gel Kit is the simplest option for the purification of PCR products and for the isolation of DNA fragments from TAE and TBE agarose gel slices. A fast and easy-to-follow protocol is given for each application.
PCR products can be purified in 10 minutes using simple binding and elution steps. Concentrated PCR products ranging between 60 bp and 15 kb can be eluted, removing primers, nucleotides, enzymes, mineral oil, salts and other impurities.
DNA fragments between 50 bp and 20 kb can be extracted from agarose gel slices in 20 minutes using a color indicator to help maintain optimal pH and identify undissolved agarose.
ISOLATE II PCR and Gel Kit has been designed to deliver optimal performance in downstream applications, including transformations, cloning, sequencing and restriction analysis.
Applications
- Cloning
- Ligation
- Restriction digestion
- Fluorescence sequencing
- Labeling
- PCR
- Transfection
- In vitro transcription

Nucleic Acid Isolation Guide
Download the ISOLATE II Guide with detailed product descriptions and performance data to help you choose the best product for your researchISOLATE II Selection Chart
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试剂盒里有详细的说明书,告诉你样品需要多少量,每个试剂需要加入多少量,和详细的实验步骤,一般买来就可以用,不用人教。
所以你问一个样需要多少量是没法回答的,测定过程是要加很多种试剂的。
1.模板提取(一般为RNA):Trizol、氯仿、异丙醇、无水乙醇、DEPC处理水
2.模板浓度测定:分光光度计或NanoDrop
3.逆转录:逆转录试剂盒(或者一步法试剂盒),这一步可以用普通PCR做,也可以用水域做。
4.荧光定量PCR试剂:通常有用SYBR Green Mix做的,但是这里建议你用EvaGreen做,灵敏度和平行性都要好于SYBR Green,并且如果你那是ABI或者Stratagene的PCR如果用SYBR Green还需要加一步Rox很麻烦。
5.其他:除了以上的那些还需要离心管、PCR管或板(Axygen反应比较好)、移液枪等,暂时就想到这么多。
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只要引物设计的可以在基因组上做,那么理论上是没问题的!
只需要用RT-PCR荧光定量试剂盒里面的第二个,专门用来做Real-timePCR的那些试剂就可以了!

