
The products listed on this page are being phased out. Replacement products are already available:
We recommend replacing Taq full DNA polymerase with the newer TaKaRa Taq DNA polymerase, which is a recombinant version of the thermostable, full-length Taq. Takara Taq is available as individual components, in a hot-start formulation, or as a complete kit.
The products listed on this page are being phased out. Replacement products are already available:We recommend replacing Taq full DNA polymerase with the newer TaKaRa Taq DNA polymerase, which is a recombinant version of the thermostable, full-length Taq. Takara Taqpolymerase is available as individual components, in a hot-start formulation, or as a complete kit.
Taqfull DNA polymerase is an ultra-pure, highly efficient, full-length, recombinant version of the Thermus aquaticus (Taq) YT1 DNA Polymerase (94 kD) (Engelke et al. 1990). This enzyme possesses two important catalytic activities: a 5"→3" polymerase activity, and a double-strand-specific 5"→3" exonuclease activity. Like other full-length Taq DNA Polymerases, this enzyme lacks 3"→5" exonuclease (proofreading) activity. This enzyme is suitable for any general PCR amplification procedure. It amplifies even rare targets from bacterial and plasmid DNA, cDNA, and complex genomic DNA.
Taqfull DNA polymerase is available in several formats:
- Individual components of polymerase enzyme mix (Cat. # 639233 and 639234)—separate tubes ofTaqfull DNA polymerase and 10X buffer
- Taq full DNA polymerase in a complete kit (Cat. # 639235)—includes Taq, 10X buffer, MgCl2, dNTPs, control genomic DNA template, and primer mix
- A hot-start polymerase formulation in a complete kit (Cat. # 639231)—includes hot-start Taq, 10X buffer, MgCl2, dNTPs, control genomic DNA template, and primer mix
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谢谢谢
1.模板提取(一般为RNA):Trizol、氯仿、异丙醇、无水乙醇、DEPC处理水
2.模板浓度测定:分光光度计或NanoDrop
3.逆转录:逆转录试剂盒(或者一步法试剂盒),这一步可以用普通PCR做,也可以用水域做。
4.荧光定量PCR试剂:通常有用SYBR Green Mix做的,但是这里建议你用EvaGreen做,灵敏度和平行性都要好于SYBR Green,并且如果你那是ABI或者Stratagene的PCR如果用SYBR Green还需要加一步Rox很麻烦。
5.其他:除了以上的那些还需要离心管、PCR管或板(Axygen反应比较好)、移液枪等,暂时就想到这么多。
两个意义不一样啊,定性是确定你抗体是阴还是阳的,如果阳证明2个情况,一个是你感染了丙肝病毒,另一个是你曾经感染过这个病毒,现在已经好了。但是到底是哪个情况还要进一步做定量测试既病毒RNA检测。如果这个测试值在最低线以下就证明你现在没事,不具有传染性也不是患者只是携带者;如果高于最低线那你就是患者了,就需要治疗
1.模板提取(一般为RNA):Trizol、氯仿、异丙醇、无水乙醇、DEPC处理水
2.模板浓度测定:分光光度计或NanoDrop
3.逆转录:逆转录试剂盒(或者一步法试剂盒),这一步可以用普通PCR做,也可以用水域做。
4.荧光定量PCR试剂:通常有用SYBR Green Mix做的,但是这里建议你用EvaGreen做,灵敏度和平行性都要好于SYBR Green,并且如果你那是ABI或者Stratagene的PCR如果用SYBR Green还需要加一步Rox很麻烦。
5.其他:除了以上的那些还需要离心管、PCR管或板(Axygen反应比较好)、移液枪等,暂时就想到这么多。
3.逆转录:逆转录试剂盒(或者一步法试剂盒),这一步可以用普通PCR做,也可以用水域做。
4.荧光定量PCR试剂:通常有用SYBR Green Mix做的,但是这里建议你用EvaGreen做,灵敏度和平行性都要好于SYBR Green,并且如果你那是ABI或者Stratagene的PCR如果用SYBR Green还需要加一步Rox很麻烦。
5.其他:除了以上的那些还需要离心管、PCR管或板(Axygen反应比较好)、移液枪等,暂时就想到这么多。
加入荧光标记探针,巧妙地把核算扩增、杂交、光谱分析和实时检测技术结合在一起,借助于荧光信号来检测PCR产物。一方面提高了灵敏度,另一方面还可以做到PCR每循环一次就收集一个数据,建立实时扩增曲线,准确地确定CT值,从而根据CT值确定起始DNA的拷贝数,做到真正意义上的DNA定量。另外由于CT值是一个完全客观的参数,CT值越小,模版DNA的起始拷贝数越小。因此,利用CT值确定DNA拷贝数实时PCR方法比普通终点定量方法更加准确

