
The products listed on this page are being phased out. Replacement products are already available:
We recommend replacing Taq full DNA polymerase with the newer TaKaRa Taq DNA polymerase, which is a recombinant version of the thermostable, full-length Taq. Takara Taq is available as individual components, in a hot-start formulation, or as a complete kit.
The products listed on this page are being phased out. Replacement products are already available:We recommend replacing Taq full DNA polymerase with the newer TaKaRa Taq DNA polymerase, which is a recombinant version of the thermostable, full-length Taq. Takara Taqpolymerase is available as individual components, in a hot-start formulation, or as a complete kit.
Taqfull DNA polymerase is an ultra-pure, highly efficient, full-length, recombinant version of the Thermus aquaticus (Taq) YT1 DNA Polymerase (94 kD) (Engelke et al. 1990). This enzyme possesses two important catalytic activities: a 5"→3" polymerase activity, and a double-strand-specific 5"→3" exonuclease activity. Like other full-length Taq DNA Polymerases, this enzyme lacks 3"→5" exonuclease (proofreading) activity. This enzyme is suitable for any general PCR amplification procedure. It amplifies even rare targets from bacterial and plasmid DNA, cDNA, and complex genomic DNA.
Taqfull DNA polymerase is available in several formats:
- Individual components of polymerase enzyme mix (Cat. # 639233 and 639234)—separate tubes ofTaqfull DNA polymerase and 10X buffer
- Taq full DNA polymerase in a complete kit (Cat. # 639235)—includes Taq, 10X buffer, MgCl2, dNTPs, control genomic DNA template, and primer mix
- A hot-start polymerase formulation in a complete kit (Cat. # 639231)—includes hot-start Taq, 10X buffer, MgCl2, dNTPs, control genomic DNA template, and primer mix
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1.步骤简单,45分钟内完成测定,比经典Lowry法快4倍而且更加方便。
2.灵敏度高,检测浓度下限达到25μg/ml,最小检测蛋白量达到0.5μg,待测样品体积为1-20μl 。
3.BCA法测定蛋白浓度不受绝大部分样品中的去污剂等化学物质的影响,可以兼容样品中高达5%的SDS,5%的Triton X-100,5%的Tween 20,60,80。
4.在20-2000μg/ml浓度范围内有良好的线性关系。
5.检测不同蛋白质分子的变异系数远小于考马斯亮蓝法蛋白定量。
两个意义不一样啊,定性是确定你抗体是阴还是阳的,如果阳证明2个情况,一个是你感染了丙肝病毒,另一个是你曾经感染过这个病毒,现在已经好了。但是到底是哪个情况还要进一步做定量测试既病毒RNA检测。如果这个测试值在最低线以下就证明你现在没事,不具有传染性也不是患者只是携带者;如果高于最低线那你就是患者了,就需要治疗
1.模板提取(一般为RNA):Trizol、氯仿、异丙醇、无水乙醇、DEPC处理水
2.模板浓度测定:分光光度计或NanoDrop
3.逆转录:逆转录试剂盒(或者一步法试剂盒),这一步可以用普通PCR做,也可以用水域做。
4.荧光定量PCR试剂:通常有用SYBR Green Mix做的,但是这里建议你用EvaGreen做,灵敏度和平行性都要好于SYBR Green,并且如果你那是ABI或者Stratagene的PCR如果用SYBR Green还需要加一步Rox很麻烦。
5.其他:除了以上的那些还需要离心管、PCR管或板(Axygen反应比较好)、移液枪等,暂时就想到这么多。
谢谢谢
3.逆转录:逆转录试剂盒(或者一步法试剂盒),这一步可以用普通PCR做,也可以用水域做。
4.荧光定量PCR试剂:通常有用SYBR Green Mix做的,但是这里建议你用EvaGreen做,灵敏度和平行性都要好于SYBR Green,并且如果你那是ABI或者Stratagene的PCR如果用SYBR Green还需要加一步Rox很麻烦。
5.其他:除了以上的那些还需要离心管、PCR管或板(Axygen反应比较好)、移液枪等,暂时就想到这么多。
(1)已包被抗原或抗体的固相载体(免疫吸附剂);
(2)酶标记的抗原或抗体(结合物);
(3)酶的底物;
(4)阴性对照品和阳性对照品(定性测定中),参考标准品和控制血清(定量测定中);
(5)结合物及标本的稀释液;
(6)洗涤液,在板式ELISA中,常用的稀释液为含0.05%吐温20磷酸缓冲盐水;
(7)酶反应终止液,常用的HRP反应终止液为硫酸,其浓度按加量及比色液的最终体积而异,在板式ELISA中一般采用3mol/L。向左转|向右转
临床实验室对商品定量试剂盒分析性能的验证.pdf(17431.6k)

