Single-cell transcriptomics is important in understanding how gene expression of individual cells varies within cell populations. We have developed an end-capture method that focuses the majority of sequencing data on the 3" ends of mRNA transcripts from single cells. This enables you to collect high quality data with fewer sequencing reads. This kit incorporates features from the industry-leading SMART-Seq v4 kit, including SMART (SwitchingMechanismat 5" End of RNATemplate) and locked nucleic acid (LNA) technology.
Single-cell transcriptomics is important in understanding how gene expression of individual cells varies within cell populations. We have developed an end-capture method that focuses the majority of sequencing data on the 3" ends of mRNA transcripts from single cells. This enables you to collect high quality data with fewer sequencing reads. This kit incorporates features from the industry-leading SMART-Seq v4 kit, including SMART (SwitchingMechanismat 5" End of RNATemplate) and locked nucleic acid (LNA) technology.
In addition to focusing reads on the 3" end of mRNA transcripts, this kit also includes in-line indexes that serve as cell barcodes on Read 2 that enable pooling of up to 12 samples per Illumina index pair, resulting in up to 1,152 samples per sequencing lane. This pooling further reduces the time and costs of discovery. This kit takes a convenient input volume of 1–10.5 µl and relies on the Illumina® Nextera® XT DNA Library Preparation Kit. This kit has been designed and validated to prepare cDNA samples and subsequent sequencing libraries for the following Illumina sequencing platforms: HiSeq®, NextSeq®, MiSeq®, and MiniSeq™.
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试剂盒里有详细的说明书，告诉你样品需要多少量，每个试剂需要加入多少量，和详细的实验步骤，一般买来就可以用，不用人教。
所以你问一个样需要多少量是没法回答的，测定过程是要加很多种试剂的。
只要引物设计的可以在基因组上做，那么理论上是没问题的！
只需要用RT-PCR荧光定量试剂盒里面的第二个，专门用来做Real-timePCR的那些试剂就可以了！
3.逆转录：逆转录试剂盒（或者一步法试剂盒），这一步可以用普通PCR做，也可以用水域做。
4.荧光定量PCR试剂：通常有用SYBR Green Mix做的，但是这里建议你用EvaGreen做，灵敏度和平行性都要好于SYBR Green，并且如果你那是ABI或者Stratagene的PCR如果用SYBR Green还需要加一步Rox很麻烦。
5.其他：除了以上的那些还需要离心管、PCR管或板（Axygen反应比较好）、移液枪等，暂时就想到这么多。
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