Description
MyTaq™ Plant-PCR Kit offers fast, highly-specific, direct PCR from a wide range of plant leaf samples. The novel buffer system circumvents the need for additional PCR stabilizers and traditional purification steps, thereby avoiding a complex extraction process.
Product Highlights
- Fast- eliminate complex, slow and costly DNA extraction steps, thereby reducing time to results
- Sensitive – incorporates MyTaq HS DNA Polymerase that exhibits increased affinity for DNA, thereby improving yield of even the most challenging targets
- Robust- specially developed to overcome common plant-derived PCR inhibitors such as polyphenolics and polysaccharides for highest PCR success rates and improved sensitivity
- Versatile- perfect for a wide range of plant species, avoiding the need for further optimization, thereby minimizing setup time and reducing cost
- Simple- ideal for fast genotyping in plant genetic studies, mutation detection, confirming transgenic plant and knockout analysis
Product Description
MyTaq™ Plant PCR Kit is recommended for fast, specific and direct PCR from a wide range of plant leaf samples. MyTaq Plant-PCR Kit incorporates MyTaq HS DNA Polymerase, a next-generation hot-start polymerase that delivers highly-specific PCR amplification. Furthermore, MyTaq HS has an increased affinity for template DNA, giving a high PCR product yield for most challenging templates. MyTaq HS DNA Polymerase has been developed to give more robust amplification than other commonly-used polymerases meaning it performs reliably even in the presence of PCR inhibitors.
MyTaq Plant-PCR Kit includes novel buffer system that replaces the need for complicated extraction or purification steps, including freezing of plant tissues with liquid nitrogen, mechanical disruption, organic extraction or column DNA purification. The advanced formulation of MyTaq Plant-PCR Kit also allows fast cycling conditions to be used, without compromising PCR specificity and yield.
MyTaq Plant-PCR Kit has been developed to tolerate the PCR inhibitors typically present in plant samples, including polyphenolics and polysaccharides, thereby delivering significantly improved assay sensitivity and reproducibility. MyTaq Plant-PCR Kit increases amplification success rates from different plant types and its speed and high specificity makes it ideal for high-throughput genotyping assays.
Meridian R&D has obtained successful results when using the MyTaq Plant-PCR Kit to conduct direct PCR from a very broad range of species.These include tomato, rice, sugarcane, maize, potato, soya, wheat, barley, cashew, oak, dwarf umbrella tree, Australian laurel, red-fruit saw-sedge, blueberry lily, she-oaks, coastal rosemary, burrawang, eucalyptus/gum tree, seaweed/crayweed, coastal banksia, bottle brush and honey gem.For more information, please inquire.
As part of our test and review campaign, many of our customers have shared their positive experiences of the MyTaq Plant-PCR Kit.Our customers have also reported success for direct PCR from wheat, rosemary, cotton, tobacco, citrus, kangkong, mousear cress (Arabidopsis), cranberry, primrose, blueberry, American bellflower and several grass and fungus species.
Applications
- Genotyping for plant genetic study
- Mutation detection
- Transgenic detection
- Knockout analysis
MyTaq™ Plant-PCR Kit Workflow
Dr. Steve Hawkins delivers a simple protocol for Direct Plant PCR using MyTaq™ Plant-PCR Kit.Introduction to MyTaq
Overview, features and benefits of the MyTaq product familyPoster
A Powerful Tool for Plant Genome Analysis.PCR Enzyme Guide
Download the PCR Enzyme Guide with detailed product descriptions and performance data to help you choose the best product for your researchPCR Selection Chart
Select the best reagent for your researchebiomall.com
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我使用的是北京北方生物技术研究所提供的KIT.盒子里面有睾酮标准品，兔抗睾酮抗体，带碘标记的睾酮，和驴抗兔免疫分离剂。实验过后我查了一下生物化学书，发现睾酮这种激素的化学本质非蛋白质，而是缁醇。这样问题就出来了：据我所知，产生免疫反应的是蛋白质，然而性类固醇激素的化学本质是缁醇。请问各位，缁醇也能产生免疫反应吗？
一般来说，试剂盒曲线上的最小浓度是比较准确的，低于这个值因为与曲线是个“S"型结构有关，所以结果是有偏差的，是一种估算。再加上实验误差，所以达不到理想的效果。建议选择试剂盒时，应该看曲线上最小的浓度值，而不是试剂盒上写的灵敏度。
虽然酶活性调节ELISA方法的灵敏度目前并不十分理想，但在酶活性放大ELISA中，检测的灵敏度远比RIA高。根据质量作用定律。即免疫反应所形成的免疫复合物量与反应物浓度成正比。推测所检测的待测物分子数为1。已知1个摩尔浓度含6.02×1023个分子，那么理论推测酶活性放大ELISA方法的最低检测限可达1.7×10-24mol/L。虽然在实际应用中由于反应条件和试剂纯度以及仪器精度等因素的影响，往往达不到这个水平(大于104个分子)，但表明ELISA在灵敏度方面的改进潜力是很大的。
