
Description
Product Highlights
- Fast – streamlined protocol for reliable recovery of genomic DNA in as little as 30 minutes
- High-performance – extraction of high-quality DNA, ideal for use in all downstream applications
- Efficient – optimized lysis conditions and column matrix for improved recovery of genomic DNA from a wide range of plant samples
- Versatile – a choice of two lysis buffers ensures excellent genomic DNA recovery from any plant material
- Convenient – kit includes all necessary components, including filters (shredders) and RNase A
- Safe - no hazardous phenol/chloroform extraction
Product Description
By combining the stringency of CTAB lysis with the speed and ease-of-use of silica-membrane purification, ISOLATE II Plant DNA Kit provides a fast method for the purification of high-quality genomic DNA from most plant cells, particularly those rich in polysaccharides, including leaves, bark, roots and fruits as well as dung, animal-fecal, soil and compost samples.
Some plant tissues do not lyse well in CTAB and so an SDS-based lysis buffer is also provided as an alternative. Residual amounts of RNA remaining can be removed using RNase treatment during lysis with the RNase A supplied in the kit.
ISOLATE II Plant DNA Kit shows excellent recovery of plant DNA when different homogenization techniques are used. The silica membrane in the columns is optimized to improve DNA binding to give high-yields of high-quality DNA, even from difficult samples such as freeze-dried budding leaves. The extracted DNA is ready to use for subsequent downstream reactions such as PCR.
ISOLATE II Plant DNA Kit has been designed to deliver optimal performance in qPCR in tandem with SensiFAST Real-Time PCR Kits and in end-point PCR with MyTaq DNA Polymerase.
Applications
- qPCR
- End-point PCR
- Southern, dot and slot blotting
- Genotyping
- Restriction digestion
- Next generation sequencing
- Bisulfite conversion/methylation analysis

Nucleic Acid Isolation Guide
Download the ISOLATE II Guide with detailed product descriptions and performance data to help you choose the best product for your researchISOLATE II Selection Chart
Application Note
ISOLATE II Plant DNA Kitebiomall.com






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一般来说,试剂盒曲线上的最小浓度是比较准确的,低于这个值因为与曲线是个“S"型结构有关,所以结果是有偏差的,是一种估算。再加上实验误差,所以达不到理想的效果。建议选择试剂盒时,应该看曲线上最小的浓度值,而不是试剂盒上写的灵敏度。
虽然酶活性调节ELISA方法的灵敏度目前并不十分理想,但在酶活性放大ELISA中,检测的灵敏度远比RIA高。根据质量作用定律。即免疫反应所形成的免疫复合物量与反应物浓度成正比。推测所检测的待测物分子数为1。已知1个摩尔浓度含6.02×1023个分子,那么理论推测酶活性放大ELISA方法的最低检测限可达1.7×10-24mol/L。虽然在实际应用中由于反应条件和试剂纯度以及仪器精度等因素的影响,往往达不到这个水平(大于104个分子),但表明ELISA在灵敏度方面的改进潜力是很大的。
现在急用,但犹豫中,不知道他们的药盒效果怎样。有没有哪位同学用过啊?大家做放免的话是不是都是用解放军总医院的,还有别的好的研究所推荐吗?谢谢了

