The transfection reagent contained within Xfect Single Shots is the same as that offered inXfect Transfection Reagent, except that it has been accurately prealiquoted and lyophilized with its buffer into single tubes. Xfect Transfection Reagent has been proven to enable highly efficient plasmid transfection of a number of cell types, including primary cells and stem cells, without concomitant cellular toxicity.
The transfection reagent contained within Xfect Single Shots is the same as that offered in Xfect Transfection Reagent, except that it has been accurately prealiquoted and lyophilized with its buffer into single tubes. Xfect Transfection Reagent has been proven to enable highly efficient plasmid transfection of a number of cell types, including primary cells and stem cells, without concomitant cellular toxicity. This is because, unlike other transfection products, Xfect Transfection Reagent contains a biodegradable cationic polymer that binds to DNA, resulting in the formation of nanocomplexes that are quickly and efficiently taken up by the cell. Because the Xfect Transfection Reagent is serum-compatible, cells do not become stressed and apoptotic due to the removal of serum or the addition of serum-free medium.
The midi kit (Cat. # 631366) is designed for transfecting plasmids into cells grown in a well of a 6-well plate, and the maxi kit (Cat. # 631447) is designed for transfecting plasmids into cells grown in a 10-cm dish.
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一般来说，试剂盒曲线上的最小浓度是比较准确的，低于这个值因为与曲线是个“S"型结构有关，所以结果是有偏差的，是一种估算。再加上实验误差，所以达不到理想的效果。建议选择试剂盒时，应该看曲线上最小的浓度值，而不是试剂盒上写的灵敏度。
虽然酶活性调节ELISA方法的灵敏度目前并不十分理想，但在酶活性放大ELISA中，检测的灵敏度远比RIA高。根据质量作用定律。即免疫反应所形成的免疫复合物量与反应物浓度成正比。推测所检测的待测物分子数为1。已知1个摩尔浓度含6.02×1023个分子，那么理论推测酶活性放大ELISA方法的最低检测限可达1.7×10-24mol/L。虽然在实际应用中由于反应条件和试剂纯度以及仪器精度等因素的影响，往往达不到这个水平(大于104个分子)，但表明ELISA在灵敏度方面的改进潜力是很大的。
现在急用，但犹豫中，不知道他们的药盒效果怎样。有没有哪位同学用过啊？大家做放免的话是不是都是用解放军总医院的，还有别的好的研究所推荐吗？谢谢了
