| Kitsize | 96 |
| Method | RIA(CT) |
| Incubationtime | 1x2h(37°C) |
| Standardrange | 0.12-36ng/mL |
| Specimen/Volumes | 50µLSerum |
| Substrate/isotope | 125I<220kBq |
| RegulatoryStatus: | EU:CE |
RadioimmunoassayfortheinvitroquantitativemeasurementofhumanProgesteroneinserum.
ProgesteroneisaC-21steroidhormone(molecularweight:314.5)whichissynthesizedfromcholesterolviapregnenoloneinthegranulosaandthecacellsofthecorpusluteumundertheinfluenceofLH.Themajorproductionsitesareovaryandplacentaandsomewhattheadrenalcortexinbothmenandwomen.Progesteroneisrapidlymetabolizedintheliver.Bloodlevelsareverylowduringthefollicularphasewhereasonedoesobserveasharplyincreaseduringthelutealphaseofmenstrualcyclesreachingamaximumsome5to10daysafterthemidcycleLHpeak.
Clinicalapplications
Serumprogesteronelevels,whicharelowduringthefollicularphase,increaseduringthelutealphaseofmenstrualcycle.Unlesspregnancyoccurs,theprogesteroneleveldeclines4daysbeforethenextmenstrualperiod.Thus,themeasurementofprogesteronelevelsconstitutesawell-establishedmethodfordetectionofovulation.Buttherearemanycaseswheretheprogesteronemeasurementsarealsoofinterest:
- Tochecktheeffectivenessofovulationinduction;
- Tomonitortheembryotransferandprogesteronereplacementtherapy;
- Todetectthepatientsatriskforabortionduringthebeginningofpregnancy;
- Toaidinthediagnosticofectopicpregnancy;
- Todetectallovariantumor(benignandmalign)inpostmenopausalwomen;
- Todiagnoseluteinizedunrupturedfolliclebythedosageof17beta-estradiolandprogesteronelevelsinperitonealfluid;
- ThesteroidprofilesoffollicularfluidsandtheratioofE2/PROGallowdetectinganormaloradysfunctionalovulationinduction.(Theemptyfollicularsyndromemayreflectadysfunctionalovulationinduction).
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人FSH放免测试单位浓度为mIU/ml,可否换算成ng/ml,如何换算
非常感谢
我使用的是北京北方生物技术研究所提供的KIT.盒子里面有睾酮标准品,兔抗睾酮抗体,带碘标记的睾酮,和驴抗兔免疫分离剂。实验过后我查了一下生物化学书,发现睾酮这种激素的化学本质非蛋白质,而是缁醇。这样问题就出来了:据我所知,产生免疫反应的是蛋白质,然而性类固醇激素的化学本质是缁醇。请问各位,缁醇也能产生免疫反应吗?

