| Kitsize | 100 |
| Method | RIA |
| Incubationtime | 1x2h,1x15min |
| Standardrange | negativeandpositvecontrol |
| Specimen/Volumes | 100µLserum,plasma |
| Substrate/isotope | 125I<74kBq |
| RegulatoryStatus: | EU:CE |
Radioimmunoassayfortheinvitrosemi-quantitativemeasurementofhumanfreeanti-insulinantibodies(AIA)inserumandplasma.
A.BIOLOGicalactivity
Thepresenceofcirculatinganti-insulinantibodies(AIA)indiabeticstreatedwithinsulinhasbeenrecognizedasearlyas1955.Thehighlypurifiedinsulinpreparations,presentlyavailable,arelessimmunogenicthansomeofthepreviouslyused,lesspure,preparations.Bovineinsulinismoreimmunogenicthantheporcinehormone.Also,ithasrecentlybeenrecognizedthatAIAmaydevelop,inpatientstreatedwithhumaninsulin.Thedeterminationofcirculatinganti-insulinantibodiesisofclinicalimportanceforthefollowingreasons:
- Thepresenceoffreeanti-insulinantibodiesinplasmainterfereswiththedeterminationofinsulinbyradioimmunoassay;
- Atveryhightiters,theanti-insulinantibodiesmayinduceastateofinsulinresistance;
- Anti-insulinantibodiesmayinfluencethequalityoftheglycemiccontrol,indiabeticpatients,byprolongingthehalflifeofinsulin.
B.Clinicalapplications
- Evaluationofthepresenceoffreeanti-insulinantibodiespriortothedeterminationofinsulinlevels,byradioimmunoassay,inpatientshavingreceivedpreviousinsulintherapy;
- Evaluationofstatesofinsulinresistance;
- Adjuncttothediagnosisofsurreptitiousinsulininjections(factitiousinsulin-inducedhypoglycaemia);
- Monitoringoftheevaluationofanti-insulinantibodies,inpatientsreceivingthenewlyformulatedhumaninsulinpreparations(newcases;patientsinwhomaprevioustreatmentwithporcineorbovineinsulinisreplacedbytheadmiNISTrationofhumaninsulin).
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人FSH放免测试单位浓度为mIU/ml,可否换算成ng/ml,如何换算
非常感谢
我使用的是北京北方生物技术研究所提供的KIT.盒子里面有睾酮标准品,兔抗睾酮抗体,带碘标记的睾酮,和驴抗兔免疫分离剂。实验过后我查了一下生物化学书,发现睾酮这种激素的化学本质非蛋白质,而是缁醇。这样问题就出来了:据我所知,产生免疫反应的是蛋白质,然而性类固醇激素的化学本质是缁醇。请问各位,缁醇也能产生免疫反应吗?

